Mechanisms in epithelial-mesenchymal transition in chronic obstructive pulmonary disease

慢性阻塞性肺疾病上皮间质转化的机制

• 批准号: 20590896
• 负责人: ITO Isao
• 金额: $ 3.08万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590896/
• 关键词: II型肺胞上皮細胞; サーファクタントプロテインC; 上皮間葉移行; COPD; 気管支喘息; 気道上皮細胞; 慢性閉塞性肺疾患; 末梢気道

By adding appropriate concentration of TGF-β to cultured BEAS-2B cell, the cells exhibited epithelial-mesenchymal transition, which were enhanced by addint TLR-ligands. To perform further experiments on EMT, induced pluripotent stem cells were established from patients with COPD and inducing type II pneumocytes from iPS cells is being tried.

在培养的BEAS-2B细胞中加入适当浓度的转化生长因子-β，BEAS-2B细胞呈现上皮-间充质转化，而TLR-配体的加入可促进细胞向间质转化。为了对EMT进行进一步的实验，从COPD患者中建立了诱导多能干细胞，并正在尝试从iPS细胞诱导II型肺细胞。

项目成果

Specific IgE Response to Trichophyton and Asthma Severity

• DOI: 10.1378/chest.08-1783
• 发表时间: 2009-04-01
• 期刊: CHEST
• 影响因子: 9.6
• 作者: Matsuoka, Hirofumi;Niimi, Akio;Mishima, Michiaki
• 通讯作者: Mishima, Michiaki

Tazobactam/piperacillin for moderate-to-severe pneumonia in patients with risk for aspiration: Comparison with imipenem/cilastatin

• DOI: 10.1016/j.pupt.2010.05.007
• 发表时间: 2010-10-01
• 期刊: PULMONARY PHARMACOLOGY & THERAPEUTICS
• 影响因子: 3.2
• 作者: Ito, Isao;Kadowaki, Seizo;Mishima, Michiaki
• 通讯作者: Mishima, Michiaki

Effect of inhaled corticosteroids on small airways in asthma: Investigation using impulse oscillometry

• DOI: 10.1016/j.pupt.2009.01.005
• 发表时间: 2009-08-01
• 期刊: PULMONARY PHARMACOLOGY & THERAPEUTICS
• 影响因子: 3.2
• 作者: Yamaguchi, Masafumi;Niimi, Akio;Mishima, Michiaki
• 通讯作者: Mishima, Michiaki

Relationship between Small Airway Function and Health Status, Dyspnea and Disease Control in Asthma

• DOI: 10.1159/000242113
• 发表时间: 2010-01-01
• 期刊: RESPIRATION
• 影响因子: 3.7
• 作者: Takeda, Tomoshi;Oga, Toru;Mishima, Michiaki
• 通讯作者: Mishima, Michiaki

Lipopolysaccharide induced connective tissue growth factor gene expression in human bronchial epithelial cells.

脂多糖诱导人支气管上皮细胞结缔组织生长因子基因表达。

• 发表时间: 2010
• 期刊: Respirology 15
• 作者: Nishioka M;Ogawa E;Kinose D;Haruna A;Ohara T;Ito I;Hoshino Y;Ito Y;Matsumoto H;Niimi A;Mio T;Chin K;Hirai T;Muro S;Mishima M.
• 通讯作者: Mishima M.