Development of a Time-Resolved Infrared Spectrophotometer ～ Elucidation of the proton pumping mechanism ～

时间分辨红外分光光度计的开发 ~ 质子泵浦机制的阐明 ~

• 批准号: 20770125
• 负责人: YAMAGUCHI Satoru
• 金额: $ 2.75万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770125/
• 关键词: 時間分解測定; 赤外分光; プロトンポンプ; 赤外分光法; マイクロチップ; 生体エネルギー変換; 構造生物学; 膜蛋白質; エネルギー変換

A femtosecond infrared laser was combined to our infrared spectrometer for light source. A chamber was constructed to cover while optical system because the water vapor makes IR spectra worth remarkably and it was filled with dry N_2 gas. For time-resolved IR experiment, a micro flow cell that dimension has 50 x 10^<-6> m (light path length) and 400 x 10^<-6> m (flow pass width) was made by sandwiched SU-8 polymer between CaF_2 windows. A difference spectra between reduced state and oxidized state of cytochrome c oxidase was acquired by using these system.

红外光谱仪采用飞秒红外激光器作为光源。由于水蒸气对红外光谱的影响很大，因此在光学系统的外围设置了一个封闭的腔室，并充入干燥的N_2气体。对于时间分辨红外光谱实验，我们用SU-8聚合物夹在CaF_2窗口之间制成了一个尺寸为50 × 10 ~ 4 μ <-6>m（光路长度）和400 × 10 ~ 4 μ <-6>m（流道宽度）的微流动池。利用该系统获得了细胞色素c氧化酶还原态和氧化态的差光谱。

项目成果

Influence of Membrane Curvature on the Structure of the Membrane-Associated Pleckstrin Homology Domain of Phospholipase C-δ1

膜曲率对磷脂酶 C-δ1 膜相关 Pleckstrin 同源结构域结构的影响

• 发表时间: 2009
• 期刊: Biochimica et Biophysica Acta - Biomembrane 1788
• 作者: N. Uekama;T. Aoki;T. Maruoka;S. Kurisu;A. Hatakeyama;S. Yamaguchi;M. Okada;H. Yagisawa;K. Nishimura;S. Tuzi
• 通讯作者: S. Tuzi

Participation of the BC Loop in the Correct Folding of Bacteriorhodopsin as Revealed by Solid-state NMR

• DOI: 10.1111/j.1751-1097.2009.00536.x
• 发表时间: 2009-03-01
• 期刊: PHOTOCHEMISTRY AND PHOTOBIOLOGY
• 影响因子: 3.3
• 作者: Kawamura, Izuru;Tanabe, Junko;Naito, Akira
• 通讯作者: Naito, Akira

Red-Excitation Resonance Raman Analysis of the nFe=O Mode of Ferryl-Oxo Hemoproteins

Ferryl-Oxo 血红蛋白 nFe=O 模式的红色激发共振拉曼分析

• 发表时间: 2008
• 期刊: J. Am. Chem. Soc. 130
• 作者: Ikemura;K.;Mukai;M.;Shimada;H.;Tsukihara;T.;Yamaguchi;S.;Shinzawa-Itoh;K.;Yoshikawa;S.;Ogura;T.
• 通讯作者: T.

Dynamics change of Phoborhodopsin and transducer by activation: Study using D75N mutant of the receptor by site -directed solid-state 13C NMR

活化引起的磷紫红质和转导器的动力学变化：通过定点固态 13C NMR 使用受体的 D75N 突变体进行研究

• 发表时间: 2008
• 期刊: Photochem. Photobiol vol.84
• 作者: I. Kawamura;H. Yoshida;Y. Ikeda;S. Yamaguchi;S. Tuzi;H. Saito;N. Kamo;A. Naito
• 通讯作者: A. Naito

Spectroscopic Characterization of a Hydroperoxo-Heme Intermediate: Conversion of a Side-On Peroxo to an End-On Hydroperoxo

氢过氧血红素中间体的光谱表征：侧接过氧化合物到端接氢过氧化合物的转化

• 发表时间: 2009
• 期刊: Angewandte Chemie International Edition 48
• 作者: J.G. Liu;T. Ohta;S. Yamaguchi;T. Ogura;S. Sakamoto;Y. Maeda;Y. Naruta
• 通讯作者: Y. Naruta