Time resolved infrared spectroscopy of cytochrome c oxidase in aqueous solution

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The newly developed time-resolved infrared system was constructed using fs infrared laser for a light source to investigate a proton pumping mechanism of cytochrome c oxidase(C_cO). The function of the O_2 reduction site using CO as an analogue of O_2 was investigated by this new system which is capable of analyzing C_cO in H_2O solution. The results indicate the existence of a conformational relay system which plays a key role in connecting the CuB site and the water channel for efficient collection of protons being pumped. It was found that transient CO-binding to Cu_B, after Fe_<a3>-CO photolysis, opens the water-channel.