Analysis of chromosomal translocation in humans by using medaka germ cell and its application

青鳉生殖细胞分析人类染色体易位及其应用

• 批准号: 20770141
• 负责人: INAGAKI Hidehito
• 金额: $ 2.83万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770141/
• 关键词: 染色体転座; DNAの高次構造; DNA二重鎖切断; DNA高次構造; ゲノム; 核酸; 発生・分化; 動物; 染色体

Chromosomal translocations often cause a variety of diseases, such as cancers or infertility. To find mechanism of a frequently occurring translocation in humans as an example, I used medaka germ cells as a model. In addition, I made a translocation model system of a human cell line and made an attempt to find factors involving the translocation. As a result, I proposed a novel mechanism of the recurrent translocation. This result might lead to a new gene transfer technique in the future.

染色体易位通常会导致多种疾病，例如癌症或不孕症。为了寻找人类中频繁发生的易位机制，我使用了青鳉生殖细胞作为模型。此外，我还制作了人类细胞系的易位模型系统，并尝试寻找涉及易位的因素。因此，我提出了一种新的反复易位机制。这一结果可能会在未来催生一种新的基因转移技术。

项目成果

Impaired DNA replication prompts deletions within palindromic sequences, but does not induce translocations in human cells

• DOI: 10.1093/hmg/ddp279
• 发表时间: 2009-09-15
• 期刊: HUMAN MOLECULAR GENETICS
• 影响因子: 3.5
• 作者: Kurahashi, Hiroki;Inagaki, Hidehito;Emanuel, Beverly S.
• 通讯作者: Emanuel, Beverly S.

Choromosomal instability mediated by non-B DNA: cruciform conformation and not DNA sequence is responsible for recurrent translocation in humans

非 B DNA 介导的染色体不稳定性：十字形构象而非 DNA 序列导致人类反复易位

• 发表时间: 2009
• 期刊: Genome Res Vol.19、No.2
• 作者: Inagaki H;Ohye T;Kogo H;Kato T;Bolor H;Taniguchi M;Shaikh TH;Emanuel BS;Kurahashi H
• 通讯作者: Kurahashi H

Artemisは十字架型のパリンドローム構造を切断し染色体転座を誘発する

Artemis打破十字形回文结构并诱导染色体易位

• 发表时间: 2008
• 作者: M Tsuchiya;EF. Sato;M Inoue;and A Asada;稲垣秀人
• 通讯作者: 稲垣秀人

Artemis cleaves cruciform- forming palindromic DNA leading to recurrent translocation in humans

Artemis 切割形成十字形的回文 DNA，导致人类反复易位

• 发表时间: 2008
• 作者: Hirota;K.;Inagaki H.
• 通讯作者: Inagaki H.

Paternal origin of the de novo constitutional t(11;22)(q23;q11)

• DOI: 10.1038/ejhg.2010.20
• 发表时间: 2010-07-01
• 期刊: EUROPEAN JOURNAL OF HUMAN GENETICS
• 影响因子: 5.2
• 作者: Ohye, Tamae;Inagaki, Hidehito;Kurahashi, Hiroki
• 通讯作者: Kurahashi, Hiroki