Screening for the zebrafish mutants that exhibit the abnormal expression patterns of Hox genes.

筛选表现出Hox基因异常表达模式的斑马鱼突变体。

• 批准号: 20770172
• 负责人: KAWAMURA Akinori
• 金额: $ 2.75万
• 依托单位: Saitama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770172/
• 关键词: 発生; 突然変異体; スクリーニング; 転写制御機構; ゼブラフィッシュ; 遺伝子; 脊椎動物; 前後軸形成; 変異体; 転写制御; スクリーニング転写制御

In vertebrates, the characteristic features such as limbs and ribs were observed along the anteroposterior axis. These morphological differences along the anteroposterior axis are specified during early embryogenesis. Genetic studies in Drosophila have shown that Hox genes play a central role in this process. Several lines of accumulating evidence indicate that the identity along the anteroposterior axis is specified by the ordered expression patterns of Hox genes in Drosophila as well as vertebrates. However, the molecular mechanism how the ordered expression patterns of Hox genes along the anteroposterior axis remains poorly understood in vertebrates. In this study to isolate the mutant that exhibits the abnormal expression patterns of Hox genes. I have performed the F2 mutant screening by using zebrafish treated with ENU mutagen. So far, I identified at least 13 mutants which exhibit the abnormal development in zebrafsih. At present, I have investigated the expression patterns of several genes including Hox genes by whole-mount in situ hybridization. In addition, I proceed to perform the F2 screening for further identification of mutants.

在脊椎动物中，沿着前后轴观察到四肢和肋骨等特征。这些沿前后轴的形态差异是在早期胚胎发生过程中确定的。果蝇的遗传学研究表明，Hox 基因在此过程中发挥着核心作用。几条不断积累的证据表明，沿前后轴的同一性是由果蝇和脊椎动物中 Hox 基因的有序表达模式指定的。然而，脊椎动物中 Hox 基因沿前后轴有序表达模式的分子机制仍知之甚少。在这项研究中，我们分离出表现出 Hox 基因异常表达模式的突变体。我使用经过 ENU 诱变剂处理的斑马鱼进行了 F2 突变体筛选。到目前为止，我已经鉴定出至少 13 个在斑马鱼中表现出异常发育的突变体。目前，我通过整体原位杂交研究了包括Hox基因在内的多个基因的表达模式。此外，我继续进行F2筛选以进一步鉴定突变体。

项目成果

Ripply3-mediated restricted modulation of Tbx1 activity in the development of pharyngeal arches

Ripply3介导的咽弓发育过程中Tbx1活性的限制性调节

• 发表时间: 2009
• 作者: Okubo T;et al.,(11人中3人目)
• 通讯作者: et al.,(11人中3人目)

咽頭弓を中心に頭部に異常を示すゼブラフィッシュ突然変異体の解析

斑马鱼突变体分析显示头部异常，主要是咽弓

• 发表时间: 2009
• 作者: 吉村麻美;川村哲規;二階堂昌孝;山田浩二;新井恒;弥益恭
• 通讯作者: 弥益恭

Analysis of the zebrafish mutant with defects in the head structure and escape response.

头部结构和逃避反应缺陷的斑马鱼突变体分析。

• 发表时间: 2008
• 作者: Mami Yoshimura;Akinori Kawamura;Masataka Nikaido;Koji Yamada;Hisashi Arai;Kvo Yamasu
• 通讯作者: Kvo Yamasu

生体制御学コース発生生物学研究室HP:

生物防治课程发育生物学实验室HP：

Activator-to-repressor conversion of T-box transcription factors by the ripply family of Groucho/TLE-associated mediators

• DOI: 10.1128/mcb.01754-07
• 发表时间: 2008-05-01
• 期刊: MOLECULAR AND CELLULAR BIOLOGY
• 影响因子: 5.3
• 作者: Kawamura, Akinori;Koshida, Sumito;Takada, Shinji
• 通讯作者: Takada, Shinji