Biocontrol activity of an endophytic Streptomyces sp. strain MBCN152-1 and its mode of action

内生链霉菌的生物防治活性。

• 批准号: 20780033
• 负责人: SHIMIZU Masafumi
• 金额: $ 2.75万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20780033/
• 关键词: 内生放線菌; 生物防除; 黒すす病; 菌寄生; 放線菌; キャベツ; セル成型苗; 植物内生; Streptomyces; キャベツセル成型苗; Alfernaria; 細胞壁分解酵素

By scanning electron microscopy, it was revealed that Streptomyces sp. strain MBCN152-1 colonized cuticle surface and intercellular spaces of cabbage cotyledons. Light and fluorescent microscopic observation indicated that MBCN152-1 could discern A. brassicicola from other fungi, and parasitize hyphal cell wall of A. brassicicola. However, interestingly, MBCN152-1 exhibited intensive biocontrol effect against non-host pathogen Glomerella cingulata, causal agent of strawberry anthracnose. When cabbage seedlings colonized by MBCN152-1 were inoculated with A. brassicicola, infection by the pathogen was significantly suppressed, though formation of physical barrier such as papillae was not observed in the cells which were penetrated by infection hyphae of the pathogen. These results suggested that mycoparasitism and induction of systemic resistance without formation of physical barrier in the host plant cells were involved in the mechanisms of biocontrol by strain MBCN152-1.

扫描电镜观察表明，链霉菌MBCN 152 -1定殖于甘蓝子叶角质层表面和细胞间隙。光学显微镜和荧光显微镜观察表明，MBCN 152 -1能识别A. brassicicicola等真菌，并寄生于A.芸苔属。然而，有趣的是，MBCN 152 -1对草莓炭疽病的非寄主病原菌Glomerella cingulata表现出强烈的生防效果。MBCN 152 -1定殖的甘蓝幼苗接种A.在小油菜中，尽管在被病原体的感染菌丝穿透的细胞中没有观察到物理屏障如乳突的形成，但病原体的感染被显著抑制。这些结果表明，MBCN 152 -1菌株的生防机制可能与其在寄主细胞内不形成物理屏障的情况下，通过寄生和诱导系统抗性有关。

项目成果

Streptomyces sp. MBCN152-1株に関する研究.(1)キャベツセル成型苗黒すす病に対する生物防除活性

链霉菌MBCN152-1菌株对甘蓝细胞幼苗黑烟的生物防治活性研究。

• 发表时间: 2009
• 作者: Masafumi Shimizu;et al.;清水将文・中筋智子・中山幹康・窪田昌春・鈴木智子・山中聡
• 通讯作者: 清水将文・中筋智子・中山幹康・窪田昌春・鈴木智子・山中聡

ウリ類炭疽病に対する放線菌殺菌剤の開発〜有望内生放線菌株の探索〜

防治葫芦炭疽病的放线菌杀菌剂的开发 - 寻找有前景的内生放线菌菌株 -

• 发表时间: 2008
• 作者: Masafumi Shimizu;et al.;清水将文・中筋智子・中山幹康・窪田昌春・鈴木智子・山中聡;清水将文・矢澤佐知子・牛島悠介
• 通讯作者: 清水将文・矢澤佐知子・牛島悠介

A promising strain of endophytic Streptomyces sp for biological control of cucumber anthracnose

• DOI: 10.1007/s10327-008-0138-9
• 发表时间: 2009-02-01
• 期刊: JOURNAL OF GENERAL PLANT PATHOLOGY
• 影响因子: 1.2
• 作者: Shimizu, Masafumi;Yazawa, Sachiko;Ushijima, Yusuke
• 通讯作者: Ushijima, Yusuke

微生物と植物の相互作用-病害と生物防除-

微生物与植物之间的相互作用 - 疾病与生物防治 -

• 发表时间: 2009
• 作者: 廣瀬望;滝澤陽子;土井元章;土井元章・滝澤陽子・廣瀬望;岡田朋大;松原陽一・Nahiyan A.S.M・Louisa Robinson・Peter Jeffries;松原陽一;ナヒヤン・松原陽一;岡田朋大・松原陽一;松原陽一;岡田朋大(学生);松原陽一;松原陽一(共著)
• 通讯作者: 松原陽一(共著)

微生物と植物の相互作用:病害と生物防除(分担:内生放線菌による誘導抵抗)

微生物与植物的相互作用：疾病与生物防治（参与：内生放线菌诱导抗性）

• 发表时间: 2009
• 作者: Nobumitsu Sasaki;Takuya Ogata;Masakazu Deguchi;Shoko Nagai;Atsushi Tamai;Tetsuo Meshi;Shigeki Kawakami;Yuichiro Watanabe;Yasuhiko Matsushita;Hiroshi Nyunoya.;清水将文
• 通讯作者: 清水将文