Characterization of the PA5471 gene involved in the antibiotic inducibility of the MexXY multidrug efflux system of Pseudomonas aeruginosa.

参与铜绿假单胞菌 MexXY 多药外排系统抗生素诱导的 PA5471 基因的表征。

• 批准号: 20790079
• 负责人: MORITA Yuji
• 金额: $ 2.75万
• 依托单位: Aichi Gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790079/
• 关键词: 遺伝子; 抗生物質; 細菌

The multidrug efflux system MexXY which is one of major antimicrobial resistant determinants in Pseudomonas aeruginosa is induced via the PA5471 gene product which is inducible by ribosome-targeting antimicrobial agents. A 13-amino-acid open reading frame (PA5471.1) is c.a. 250 bp upstream of the PA5471 coding sequence and its translational repression leads transcriptional inducibility of the PA5471 (or MexXY). The transcriptional termination of the PA5471.1 was implied to be the Rho-factor dependent.

铜绿假单胞菌多药外排系统MexXY是铜绿假单胞菌主要的耐药决定因子之一，它是通过核糖体靶向抗菌药物诱导的PA5471基因产物来诱导的。13个氨基酸的开放阅读框（PA 5471.1）是c.a. PA5471编码序列上游250 bp处的转录抑制基因导致了PA5471（或MexXY）的转录诱导。PA5471.1的转录终止是Rho因子依赖性的。

项目成果

Application of an inducible system to engineer unmarked conditional mutants of essential genes of Pseudomonas aeruginosa

• DOI: 10.1016/j.mimet.2010.06.001
• 发表时间: 2010-09-01
• 期刊: JOURNAL OF MICROBIOLOGICAL METHODS
• 影响因子: 2.2
• 作者: Morita, Yuji;Narita, Shin-Ichiro;Kawamura, Yoshiaki
• 通讯作者: Kawamura, Yoshiaki

緑膿菌必須遺伝子条件的破壊株構築法の開発

铜绿假单胞菌必需基因条件破坏菌株构建方法的开发

• 发表时间: 2010
• 作者: 森田雄二;ら
• 通讯作者: ら