Analysis of the mechanism of fulminant hepatitis using a hepatitisB virus strain obtained from a patient in fulminant hepatitis outbreak

利用暴发性肝炎暴发患者乙型肝炎病毒株分析暴发性肝炎的发病机制

• 批准号: 20790483
• 负责人: JUN Inoue
• 金额: $ 2万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790483/
• 关键词: 肝臓学; ウイルス性肝炎; 肝臟学

To clarify the mechanism of fulminant hepatitis induced by hepatitis B virus (HBV), we focused on a HBV strain obtained from a patient in fulminant hepatitis outbreak, and investigated the significance of mutations found in the strain and the impact to the infected cells. As a result, we demonstrated that the HBV replicative intermediates of HBV with the mutations were increase in the cells, and HBV core protein was accumulated in the nucleus of HBV-transfected cells. Moreover, the strain had direct cytotoxicity, and a part mechanism of fulminant hepatitis was clarified.

为阐明B肝炎病毒（HBV）诱发暴发性肝炎的机制，我们对从暴发性肝炎患者中分离的一株HBV毒株进行了研究，并探讨了该毒株突变的意义及其对感染细胞的影响。结果表明，HBV复制中间产物在细胞中的表达增加，HBV核心蛋白在HBV转染细胞的细胞核中积累。该菌株具有直接的细胞毒作用，初步阐明了其致重型肝炎的部分机制。

项目成果

Emergence of multiple drug-resistant mutants of hepatitis B virus with long-term lamivudine and adefovir bitherapy.

长期拉米夫定和阿德福韦双疗法出现乙型肝炎病毒多重耐药突变体。

• 发表时间: 2009
• 作者: Inoue J;et al
• 通讯作者: et al

Enhanced intracellular retention of a hepatitis B virus strain associated with fulminant hepatitis

• DOI: 10.1016/j.virol.2009.09.028
• 发表时间: 2009-12-20
• 期刊: VIROLOGY
• 影响因子: 3.7
• 作者: Inoue, Jun;Ueno, Yoshiyuki;Shimosegawa, Tooru
• 通讯作者: Shimosegawa, Tooru

Factors involved in the development of fulminant hepatitis B: Are the mutations of hepatitis B virus implicated?

暴发性乙型肝炎的发生因素：乙型肝炎病毒的突变是否与此有关？

• 发表时间: 2009
• 期刊: Hepatol Res. 39
• 作者: Inoue J;Ueno Y;Nagasaki F;Wakui Y;Kondo Y;Fukushima K;Niitsuma H;Shimosegawa T.;Inoue J.
• 通讯作者: Inoue J.

Emergence of multiple drug-resistant mutants of hepatitis B virus with long-term lamivudine and adefovir bitherapy

长期拉米夫定与阿德福韦双药治疗出现乙型肝炎病毒多重耐药突变体

• 发表时间: 2009
• 作者: 井上淳;et al.
• 通讯作者: et al.

Cytopathic effect of hepatitis B virus obtained from fulminant hepatitis patients.

从暴发性肝炎患者获得的乙型肝炎病毒的细胞病变作用。

• 发表时间: 2008
• 作者: Inoue J;et al.
• 通讯作者: et al.