Establishment of differentiation method of iPS cell derived cardiomyocytes

我们分析了小鼠iPS细胞分化为心肌细胞的差异。对纯化的iPS来源的心肌细胞进行RT-PCR分析，发现高表达残留外源基因的iPS来源的心肌细胞的搏动效率低于表达低表达外源基因的iPS来源的心肌细胞。较高的外源转基因表达水平可能有助于降低搏动集落的发生率，从而阻止向心肌细胞分化。

We analyzed the difference of cardiomyocyte differentiation derived from mouse iPS cells. RT-PCR analysis of the purified iPS-derived cardiomyocytes revealed that iPS-derived cardiomyocytes with higher expression level of residual exogenous transgene had lower beating efficiency compared with that of iPS-derived cariomyocytes with lower expression level of residual exogenous transgene. Higher expression level of exogenous transgene may have contributed to lower incidence of beating colonies, preventing differentiation to cardiomyocytes.