Establishment of differentiation method of iPS cell derived cardiomyocytes

iPS细胞来源的心肌细胞分化方法的建立

• 批准号: 20790549
• 负责人: OHNO Yohei
• 金额: $ 2.75万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790549/
• 关键词: iPS細胞; 患者特異的iPS細胞; iPS細胞由来心筋細胞; iPS細胞由来心筋の分化誘導効率; iPS細胞由来心筋の移植効率

We analyzed the difference of cardiomyocyte differentiation derived from mouse iPS cells. RT-PCR analysis of the purified iPS-derived cardiomyocytes revealed that iPS-derived cardiomyocytes with higher expression level of residual exogenous transgene had lower beating efficiency compared with that of iPS-derived cariomyocytes with lower expression level of residual exogenous transgene. Higher expression level of exogenous transgene may have contributed to lower incidence of beating colonies, preventing differentiation to cardiomyocytes.

我们分析了小鼠iPS细胞向心肌细胞分化的差异。纯化的iPS来源的心肌细胞的RT-PCR分析显示，与iPS来源的心肌细胞相比，具有较高表达水平的残余外源性转基因的iPS来源的心肌细胞具有较低的搏动效率。外源性转基因的高表达水平可能有助于降低搏动集落的发生率，从而阻止向心肌细胞的分化。

项目成果

Molecular Characterization, Safety and Feasibility of Induced Pluripotent Stem(iPS)Cell Derived Cardiomyocytes for Heart Regenerative Therapy

用于心脏再生治疗的诱导多能干 (iPS) 细胞来源的心肌细胞的分子特征、安全性和可行性

• 发表时间: 2008
• 作者: Yohei Ohno;Shinsuke Yuasa;Takeshi Onizuka;Toru Egashira;Kenichiro Shimoji;Sung Han Yoon;Takahide Arai;Chen Hao;Tomofumi Tanaka;Fumiyuki Hattori;Toshimi Kageyama;Satoshi Ogawa;Shinya Yamanaka;Keiichi Fukuda;大野洋平
• 通讯作者: 大野洋平

Molecular characterization, safety and feasibility of induced pluripotent stem (iPS) cellderived cardiomyocytes for heart regenerative therapy

用于心脏再生治疗的诱导多能干（iPS）细胞来源的心肌细胞的分子特征、安全性和可行性

• 发表时间: 2009
• 作者: Yohei Ohno;Shinsuke Yuasa;Takeshi Onizuka;Toru Egashira;Kenichiro Shimoji;Sung Han Yoon;Takahide Arai;Chen Hao;Tomofumi Tanaka;Fumiyuki Hattori;Toshimi Kageyama;Satoshi Ogawa;Shinya Yamanaka;Keiichi Fukuda
• 通讯作者: Keiichi Fukuda

Molecular Characterization of Induced Pluripotent Stem(iPS) Cell-Derived Cardiomyocytes

诱导多能干 (iPS) 细胞来源的心肌细胞的分子表征

• 发表时间: 2009
• 作者: Yohei Ohno;Shinsuke Yuasa;Toru Egashira;Tomohisa Seki;Hisayuki Hashimoto;Shugo Toyama;Sung Han Yoon;Takahide Arai;Chen Hao;Tomofumi Tanaka;Fumiyuki Hattori;Kojiro Yae;Mitsushige Murata;Satoshi Ogawa;大野洋平
• 通讯作者: 大野洋平

Molecular characterization of induced pluripotent stem (iPS) cell- derived cardiomyocytes

诱导多能干 (iPS) 细胞来源的心肌细胞的分子表征

• 发表时间: 2010
• 作者: Yohei Ohno;Shinsuke Yuasa;Toru Egashira;Tomohisa Seki;Hisayuki Hashimoto;Shugo Toyama;Takahide Arai;Tomofumi Tanaka;Fumiyuki Hattori;Kojiro Yae;Mitsushige Murata;Satoshi Ogawa;Shinya Yamanaka;Keiichi Fukuda
• 通讯作者: Keiichi Fukuda

Molecular Characterization of Induced Pluripotent Stem (iPS) Cell-Derived Cardiomyocytes

诱导多能干 (iPS) 细胞来源的心肌细胞的分子表征

• 发表时间: 2009
• 作者: Yohei Ohno;Shinsuke Yuasa;Toru Egashira;Tomohisa Seki;Hisayuki Hashimoto;Shugo Toyama;Sung Han Yoon;Takahide Arai;Chen Hao;Tomofumi Tanaka;Fumiyuki Hattori;Kojiro Yae;Mitsushige Murata;Satoshi Ogawa
• 通讯作者: Satoshi Ogawa