Research in heart development using medaka cardiac defect mutant

利用青鳉心脏缺陷突变体进行心脏发育研究

• 批准号: 20790553
• 负责人: YOON Sunghan
• 金额: $ 2.75万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790553/
• 关键词: 分子心臓病態学; 発生遺伝; 心臓発生; 遺伝学; メダカ; ENU; 小型魚類; 変異体; 心室形成不全

Using forward genetic ENU mutagenesis screening, we identified a cardiovascular mutant, big heart tube (bht), which displayed hypomorphic ventricles and a constricted outflow tract, and terminates its development at the linear heart tube stage. Positional cloning revealed that bht resulted from a mutation in versican. Injection of a versican-specific morpholino recapitulated the cardiac phenotype of bht, suggesting that bht is a loss-of-function mutant.

使用正向遗传ENU诱变筛查，我们确定了心血管突变体，大心管（BHT），该突变体显示出肌室的肌室和狭窄的流出区，并在线性心管阶段终止其发育。位置克隆表明，BHT是由Versican突变引起的。注射versican特异性的形态杆概括了BHT的心脏表型，这表明BHT是功能丧失的突变体。

项目成果

Versican is Essential for ventricular Trabeculation and outflow Tract Formation in Medaka Heart

Versican 对于青鳉心脏心室小梁和流出道形成至关重要

• 发表时间: 2009
• 作者: Sung Han Yoon;Shinji Makino;Satoshi Ogawa
• 通讯作者: Satoshi Ogawa

Versican is Essential for Chamber Formation by Recruitment of Islet-1 Expressing Cardiac Progenitor Cells

Versican 对于通过招募表达 Islet-1 的心脏祖细胞形成心室至关重要

• 发表时间: 2010
• 作者: 河野隆志;安斉俊久;安西淳;金子英弘;眞野恵範;内藤広太郎;前川裕一郎;宮庄拓;山田晋吾;横田博;吉川勉;石坂彰敏;小川聡;尹誠漢
• 通讯作者: 尹誠漢