Clarification of the role of annexin on glucose transport

阐明膜联蛋白对葡萄糖转运的作用

• 批准号: 20790638
• 负责人: FUJISHIRO Midori
• 金额: $ 2.75万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790638/
• 关键词: エネルギー; 糖質代謝異常; 糖尿病; シグナル伝達; 細胞・組織; 蛋白質; インスリン抵抗性

Recently we have discovered a novel Akt binding protein, annexin, which is characterized by Ca2+-dependent interactions with cellular membranes. First we produced cDNA encoding human annexin by PCR with primers corresponding to sequences already reported, using the cDNA library from HEK293 cells and generated recombinant adenovirus to express human annexin. We confirmed the association of annexin with Akt using adenovirus system and then made GST-annexin fusion protein to generate the antibody specific against annexin by immunizing rabbits with this antigen. Fragments of both Akt and annexin were designed by PCR using full-length cDNAs as templates to generate GST-fused proteins.

最近，我们发现了一种新型的Akt结合蛋白Annexin，其特征在于Ca2+依赖性与细胞膜的相互作用。首先，我们使用来自HEK293细胞的cDNA文库并生成重组腺病毒来表达人膜联蛋白的cDNA文库，用PCR与已经报道的序列相对应的引物来编码人膜联蛋白。我们使用腺病毒系统证实了膜联蛋白与AKT的关联，然后通过用这种抗原对兔子进行免疫，使GST-Annexin融合蛋白通过对膜联蛋白产生特异性抗体。 Akt和Annexin的片段是由PCR设计的，使用全长cDNA作为模板来生成GST融合的蛋白质。

项目成果

AMP-ctivated protein kinase activation increases phosphorylation of glycogen synthase kinase 3beta and thereby reduces cAMP-responsive element transcriptional activity and phosphoenolpyruvate carboxykinase C gene expression in the liver

AMP 激活的蛋白激酶激活增加糖原合酶激酶 3beta 的磷酸化，从而降低肝脏中 cAMP 响应元件转录活性和磷酸烯醇丙酮酸羧激酶 C 基因表达

• 发表时间: 2008
• 期刊: J Biol Chem 223
• 作者: Horike N;Sakoda H;Kushiyama A;Ono H;Fujishiro M;et.al.
• 通讯作者: et.al.

AMP-activated protein kinase activation increases phosphorylation of glycogen synthase kinase 3beta and thereby reduces cAMP-responsive element transcriptional activity and phosphoenolpyruvate carboxykinase C gene expression in the liver.

AMP 激活的蛋白激酶激活增加糖原合成酶激酶 3β 的磷酸化，从而降低肝脏中 cAMP 响应元件的转录活性和磷酸烯醇丙酮酸羧激酶 C 基因的表达。

• 发表时间: 2008
• 期刊: J Biol Chem. 283(49)
• 作者: Horike N;Sakoda H;Kushiyama A;Ono H;Fujishiro M;Kamata H;Nishiyama K;Uchijima Y;Kurihara Y;Kurihara H;Asano T
• 通讯作者: Asano T

Hepatic overexpression of a dominant negative form of raptor enhances Akt phosphorylation and restores insulin sensitivity in K/KAy mice

• DOI: 10.1152/ajpendo.00253.2007
• 发表时间: 2008-04-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
• 影响因子: 5.1
• 作者: Koketsu, Yuko;Sakoda, Hideyuki;Asano, Tomoichiro
• 通讯作者: Asano, Tomoichiro

Macrophage foam cell formation is augmented in serum from patients with diabetic angiopathy

• DOI: 10.1016/j.diabres.2009.10.011
• 发表时间: 2010-01-01
• 期刊: DIABETES RESEARCH AND CLINICAL PRACTICE
• 影响因子: 5.1
• 作者: Cui, Xinglong;Kushiyama, Akifumi;Asano, Tomoichiro
• 通讯作者: Asano, Tomoichiro

Long-term Forskolin Stimulation Induces AMPK Activation and Thereby Enhances Tight junction Formation in Human Placental Trophoblast BeWo Cells

• DOI: 10.1016/j.placenta.2008.09.008
• 发表时间: 2008-12-01
• 期刊: PLACENTA
• 影响因子: 3.8
• 作者: Egawa, M.;Kamata, H.;Asano, T.
• 通讯作者: Asano, T.