Detection of imprinting abnormalities in rhabdomyosarcoma patients using tumor and serum DNA.

使用肿瘤和血清 DNA 检测横纹肌肉瘤患者的印记异常。

• 批准号: 20790738
• 负责人: KIKUCHI Ken
• 金额: $ 1.5万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790738/
• 关键词: 横紋筋肉腫; インプリンティング; IGF2; 血清遊離DNA; H19; 血清DNA

In RMS cell lines and RMS tumor samples, we were able to detect a methylation abnormality in the imprinting center, in other words, imprinting abnormalities in IGF2 and H19 by Melting Curve Analysis. In RMS tumor samples, it was suggested that imprinting abnormalities correlate with the expression of IGF2 mRNA. Furthermore, we established a method to detect imprinting abnormalities in IGF2 and H19 in serum DNA.

在RMS细胞系和RMS肿瘤样本中，我们可以通过熔化曲线分析检测到印迹中心的甲基化异常，即IGF2和H19的印迹异常。在RMS肿瘤样本中，印迹异常与IGF2 mRNA的表达相关。此外，我们建立了一种检测血清DNA中IGF2和H19印迹异常的方法。

项目成果

Detection of IGF2 and H19 imprinting abnormalities in rhabdomyosarcoma patients using tumor and serum DNA

使用肿瘤和血清 DNA 检测横纹肌肉瘤患者的 IGF2 和 H19 印迹异常

• 发表时间: 2008
• 作者: Ken Kikuchi;Tomoko Iehara;Hajime Hosoi
• 通讯作者: Hajime Hosoi

Restoration of p53 Pathway by Nutlin-3 Induces Cell Cycle Arrest and Apoptosis in Human Rhabdomyosarcoma Cells

• DOI: 10.1158/1078-0432.ccr-08-2955
• 发表时间: 2009-06-15
• 期刊: CLINICAL CANCER RESEARCH
• 影响因子: 11.5
• 作者: Miyachi, Mitsuru;Kakazu, Naoki;Hosoi, Hajime
• 通讯作者: Hosoi, Hajime

横紋筋肉腫患者血清遊離DNAを用いたIGF2/H19 imprinting異常の検出

使用横纹肌肉瘤患者血清游离DNA检测IGF2/H19印记异常

• 发表时间: 2008
• 作者: Miyachi M;Kakazu N;Yagyu S;Katsumi Y;Tsubai-Shimizu S;Kikuchi K;Tsuchiya K;Iehara T;Hosoi H.;菊地顕
• 通讯作者: 菊地顕