Expression of sonic hedgehog signaling for regulation of liver regeneration after massive hepatectomy of the rat liver

声波刺猬信号在大鼠肝脏大面积切除术后肝再生调控中的表达

• 批准号: 20790958
• 负责人: MORINE Yuji
• 金额: $ 2.66万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790958/
• 关键词: 肝臓外科学; ソニックヘッジホッグ; 肝再生; ソニックヘッジホッジ; アクチビン; フォリスタチン; 大量肝切除

Background : This study was conducted to clarify the possible functional involvement of Hh signaling, especially in Sonic hedgehog (Shh) in liver regeneration. Methods : Six-week-old male Wister rats were performed 70% hepatectomy (70%Hx, n=6) and 90% hepatectomy (90%Hx, n=6). The liver/body weight ratio and immunohistochemistry by anti-PCNA, anti-Shh and anti-Gli-1 antibody were evaluated their expression in each groups. Results : Hepatocytes were strongly stained by PCNA at 24 hr after Hx. Regarding non-parenchymal cells, they gradually stained by PCNA from 24hr to 72hr after Hx. Shh and Gli-1 expressions of hepatocytes were higher in 24 hr than other time point. In non-parenchymal cells, they gradually increased after Hx, and Shh and Gli-1-positive non-parenchymal cells mainly existed in zone I. Conclusions : Those findings indicated some relations between Shh pathway and liver regeneration. It is possible that they induced efficient and smooth hepatic reconstruction.

背景：本研究旨在阐明 Hh 信号传导的可能功能参与，尤其是 Sonic Hedgehog (Shh) 在肝再生中的作用。方法：对六周龄雄性 Wister 大鼠进行 70% 肝切除术（70%Hx，n=6）和 90% 肝切除术（90%Hx，n=6）。通过抗PCNA、抗Shh和抗Gli-1抗体的肝脏/体重比和免疫组化评估各组的表达。结果：Hx 后 24 小时，肝细胞被 PCNA 强烈染色。对于非实质细胞，从Hx后24小时到72小时，它们逐渐被PCNA染色。 24小时肝细胞Shh和Gli-1表达高于其他时间点。非实质细胞中，Hx后逐渐增多，Shh和Gli-1阳性非实质细胞主要存在于I区。结论：这些结果表明Shh通路与肝再生之间存在一定的关系。它们有可能诱导有效且顺利的肝脏重建。

项目成果

Severe NAFL model ratにおける虚血再灌流障害に対するMHN-02の効果

MHN-02对重度NAFL模型大鼠缺血再灌注损伤的影响

• 发表时间: 2009
• 作者: Kanamoto M;Shimada M;et al.;新居章
• 通讯作者: 新居章

ラット大量肝切除における脾臓内遺伝子発現の変化-microarrayによる網羅的解析-

大鼠大规模肝切除后脾脏基因表达的变化-使用微阵列的综合分析-

• 发表时间: 2009
• 作者: 山下芳樹;野村英毅;水野聡朗;斉藤佳菜子;北野滋久;宮田恵里;田丸智巳;日浅厚則;影山慎一;中瀬一則;片山直之;Yuji Morine;Yusuke Arakawa;Yuji Mbrine;Yusuke Arakawa;西正暁;荒川悠佑;新居章;小笠原卓
• 通讯作者: 小笠原卓

Optimal administration of taerolimus in reduced-size liver.

泰罗莫司在缩小肝脏中的最佳给药。

• 发表时间: 2009
• 期刊: Dig Dis Sei. 54
• 作者: 山下芳樹;野村英毅;水野聡朗;斉藤佳菜子;北野滋久;宮田恵里;田丸智巳;日浅厚則;影山慎一;中瀬一則;片山直之;Yuji Morine
• 通讯作者: Yuji Morine

肝虚血再還流障害に対する高圧酸素療法(HBO)の効果

高压氧治疗（HBO）对肝脏缺血再灌注损伤的影响

• 发表时间: 2009
• 作者: 山下芳樹;野村英毅;水野聡朗;斉藤佳菜子;北野滋久;宮田恵里;田丸智巳;日浅厚則;影山慎一;中瀬一則;片山直之;Yuji Morine;Yusuke Arakawa;Yuji Mbrine;Yusuke Arakawa;西正暁
• 通讯作者: 西正暁

ラット大量肝切除における脾臓内遺伝子発現の変化-microarray-

大鼠大规模肝切除后脾脏基因表达的变化-微阵列-

• 发表时间: 2009
• 作者: 山下芳樹;野村英毅;水野聡朗;斉藤佳菜子;北野滋久;宮田恵里;田丸智巳;日浅厚則;影山慎一;中瀬一則;片山直之;Yuji Morine;Yusuke Arakawa;Yuji Mbrine;Yusuke Arakawa;西正暁;荒川悠佑;新居章;小笠原卓;小笠原卓
• 通讯作者: 小笠原卓