Expression of sonic hedgehog signaling for regulation of liver regeneration after massive hepatectomy of the rat liver

声波刺猬信号在大鼠肝脏大面积切除术后肝再生调控中的表达

• 批准号: 20790958
• 负责人: MORINE Yuji
• 金额: $ 2.66万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790958/
• 关键词: 肝臓外科学; ソニックヘッジホッグ; 肝再生; ソニックヘッジホッジ; アクチビン; フォリスタチン; 大量肝切除

Background : This study was conducted to clarify the possible functional involvement of Hh signaling, especially in Sonic hedgehog (Shh) in liver regeneration. Methods : Six-week-old male Wister rats were performed 70% hepatectomy (70%Hx, n=6) and 90% hepatectomy (90%Hx, n=6). The liver/body weight ratio and immunohistochemistry by anti-PCNA, anti-Shh and anti-Gli-1 antibody were evaluated their expression in each groups. Results : Hepatocytes were strongly stained by PCNA at 24 hr after Hx. Regarding non-parenchymal cells, they gradually stained by PCNA from 24hr to 72hr after Hx. Shh and Gli-1 expressions of hepatocytes were higher in 24 hr than other time point. In non-parenchymal cells, they gradually increased after Hx, and Shh and Gli-1-positive non-parenchymal cells mainly existed in zone I. Conclusions : Those findings indicated some relations between Shh pathway and liver regeneration. It is possible that they induced efficient and smooth hepatic reconstruction.

背景：本研究旨在阐明HH信号，尤其是Sonic Hedgehog(Shh)信号在肝脏再生中可能的功能参与。方法：6周龄雄性Wistar大鼠分别行70%肝切除(70%HX，n=6)和90%肝切除(90%HX，n=6)。观察各组大鼠肝/体重比及抗增殖细胞核抗原、抗Shh、抗Gli-1抗体免疫组织化学染色结果。结果：HX后24小时，肝细胞增殖细胞核抗原呈强阳性染色。对于非实质细胞，从HX后24小时到72小时，它们逐渐被增殖细胞核抗原染色。肝细胞Shh和Gli-1的表达以24小时最高。在非实质细胞中，HX后逐渐增多，Shh和Gli-1阳性的非实质细胞主要存在于I区。结论：Shh途径与肝再生有一定关系。它们有可能诱导高效、顺畅的肝重建。

项目成果

Severe NAFL model ratにおける虚血再灌流障害に対するMHN-02の効果

MHN-02对重度NAFL模型大鼠缺血再灌注损伤的影响

• 发表时间: 2009
• 作者: Kanamoto M;Shimada M;et al.;新居章
• 通讯作者: 新居章

ラット大量肝切除における脾臓内遺伝子発現の変化-microarrayによる網羅的解析-

大鼠大规模肝切除后脾脏基因表达的变化-使用微阵列的综合分析-

• 发表时间: 2009
• 作者: 山下芳樹;野村英毅;水野聡朗;斉藤佳菜子;北野滋久;宮田恵里;田丸智巳;日浅厚則;影山慎一;中瀬一則;片山直之;Yuji Morine;Yusuke Arakawa;Yuji Mbrine;Yusuke Arakawa;西正暁;荒川悠佑;新居章;小笠原卓
• 通讯作者: 小笠原卓

Optimal administration of taerolimus in reduced-size liver.

泰罗莫司在缩小肝脏中的最佳给药。

• 发表时间: 2009
• 期刊: Dig Dis Sei. 54
• 作者: 山下芳樹;野村英毅;水野聡朗;斉藤佳菜子;北野滋久;宮田恵里;田丸智巳;日浅厚則;影山慎一;中瀬一則;片山直之;Yuji Morine
• 通讯作者: Yuji Morine

肝虚血再還流障害に対する高圧酸素療法(HBO)の効果

高压氧治疗（HBO）对肝脏缺血再灌注损伤的影响

• 发表时间: 2009
• 作者: 山下芳樹;野村英毅;水野聡朗;斉藤佳菜子;北野滋久;宮田恵里;田丸智巳;日浅厚則;影山慎一;中瀬一則;片山直之;Yuji Morine;Yusuke Arakawa;Yuji Mbrine;Yusuke Arakawa;西正暁
• 通讯作者: 西正暁

ラット大量肝切除における脾臓内遺伝子発現の変化-microarray-

大鼠大规模肝切除后脾脏基因表达的变化-微阵列-

• 发表时间: 2009
• 作者: 山下芳樹;野村英毅;水野聡朗;斉藤佳菜子;北野滋久;宮田恵里;田丸智巳;日浅厚則;影山慎一;中瀬一則;片山直之;Yuji Morine;Yusuke Arakawa;Yuji Mbrine;Yusuke Arakawa;西正暁;荒川悠佑;新居章;小笠原卓;小笠原卓
• 通讯作者: 小笠原卓