Induction of pyramidal neuronal cells from bone marrow stromal stemcells and application for autologous transplantation against the cerebral infarction.

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We demonstrated the way to induce the EACC1(glutamate transpoter)-positive pyramidal neuron-like cells from rat bone marrow stromal stem cells (BMSC). Transient hypoxia and cytokines stimulation following the genes transfection of Notch intracellular domain (NICD) and pituitary adenylate cyclase activating polypeptide (PACAP) promoted the transdifferentiation of BMSCs into the mutipolar neuron-like cells. One month after the transplantation of these cells into the infracted brain, some of recipient cells had survived in the peri-ischemic area of host brain and expressed the synapse-associated proteins.