Role of Actin associated cell skeletal protein in peripheral nerve regeneration-CLP36 interaction with palladin-

CLP 36是α-辅肌动蛋白相关LIM蛋白（ALP）/enigma蛋白家族的成员，在周围神经系统中的神经突生长中起作用。我们进行了酵母双杂交筛选，鉴定出palladin为CLP 36结合蛋白。免疫共沉淀研究表明，CLP 36和palladin在背根神经节（DRG）中形成复合物。此外，CLP 36和palladin共定位于原代DRG神经元的神经突和细胞体中。此外，坐骨神经切断增加了DRG神经元中CLP 36和palladin mRNA的表达，其中CLP 36 mRNA的增加更为突出。这意味着CLP 36在神经再生中具有比palladin更特异的作用。我们的研究结果表明，CLP 36可能与palladin相互作用，影响神经突起的生长

CLP36, a member of the alpha-actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. we had performed yeast two-hybrid screening, identified palladin as a CLP36-binding protein. A coimmunoprecipitation study showed that CLP36 and palladin formed a complex in the dorsal root ganglion (DRG). In addition, CLP36 and palladin were colocalized in the neurites and cell bodies of primary DRG neurons. Furthermore, sciatic nerve transection increased the expression of both CLP36 and palladin mRNAs in DRG neurons, with the increase in CLP36 mRNA being more prominent. This implies that CLP36 has a more specific role in nerve regeneration than palladin. Our results suggest that CLP36 may interact with palladin to influence neurite outgrowth