Analysis of cytokine secretion and its application using macrophage specific syntaxin 3 knockout mice

巨噬细胞特异性突触蛋白3基因敲除小鼠细胞因子分泌分析及其应用

• 批准号: 20791063
• 负责人: IRIUCHIJIMA Nobuhisa
• 金额: $ 2.66万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791063/
• 关键词: シンタキシン3; ノックアウトマウス; syntaxin3; TNFα; コンディショナルノックアウト

Using macrophage specific syntaxin 3 knockout mice, whether syntaxin 3 was involved in the secretion of TNFα, it was evaluated by staining and ELISA. Unlike the initial estimates,the syntaxin 3 was not found to be involved in the secretion of TNFα. However, syntaxin 3 to move from the Golgi apparatus to the cell periphery, It was thought it is involved in the secretion of other cytokines.

采用巨噬细胞特异性syntaxin 3基因敲除小鼠，通过染色和ELISA检测syntaxin 3是否参与TNFα的分泌。与最初的估计不同，syntaxin 3未被发现参与TNFα的分泌。然而，syntaxin 3从高尔基体移动到细胞外周，人们认为它参与了其他细胞因子的分泌。