Identification and analysis of human spermatogonial stem cells
Identification and analysis of human spermatogonial stem cells
批准号:
20791127
负责人:
KOBAYASHI Hideyuki
金额:
$2.83万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2009
中文摘要
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英文摘要
Isolated cells from testicular tissues were maintained in serum-free medium, selected growth factors, and STO feeder layer for 1 week. We found that magnetic-activated cell sorting (MACS) was an efficient method to obtain Thy-1+ cells from testis cell suspensions. Enriched Thy-1+ cells were present among unretained cells. Thy-1+ cells were expressed human embryonic stem cell genes. Sixteen weeks after transplanting the Thy-1+ cells into nude mice, there was no evidence of teratoma.
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The characterization and function of male germline stem cells obtained from human testicular tissue
从人类睾丸组织中获得的雄性生殖干细胞的特征和功能
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[Kobayashi H, Nagao K, Nakajima K, Miura K, Ishii N, Hideyuki Kobayashi, 小林秀行]
通讯作者:
小林秀行
ヒト精巣組織からのヒト精子幹細胞human spermatogonial stem cells(SSCs)の分離及び培養の試み
尝试从人类睾丸组织中分离和培养人类精原干细胞(SSC)
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[Kobayashi H, Nagao K, Nakajima K, Miura K, Ishii N, Hideyuki Kobayashi, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行]
通讯作者:
小林秀行
ヒト精巣組織か照のヒト精子幹細胞human spermatogonial stem cells(SSCs)の分離及び培養の試み
尝试从人类睾丸组织中分离和培养人类精原干细胞(SSC)
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[Kobayashi H, Nagao K, Nakajima K, Miura K, Ishii N, Hideyuki Kobayashi, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行]
通讯作者:
小林秀行
ヒト精巣組織からのヒト精子幹細胞human spermatogonial stem cells(SSCs)の分離の試み及び機能解析
从人类睾丸组织中分离人类精原干细胞(SSC)的尝试和功能分析
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[Kobayashi H, Nagao K, Nakajima K, Miura K, Ishii N, Hideyuki Kobayashi, 小林秀行, 小林秀行, 小林秀行, 小林秀行]
通讯作者:
小林秀行
ヒト精巣組織からのヒト精子幹細胞human spermatogonial stem cells(SSCs)の分離の試み
尝试从人类睾丸组织中分离人类精原干细胞(SSC)
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[Kobayashi H, Nagao K, Nakajima K, Miura K, Ishii N, Hideyuki Kobayashi, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行]
通讯作者:
小林秀行
共 7 条
Elucidation of male infertility by epigenome analysis of iPS cells derived from male infertility and stem cell research
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Regulatory mechanism of aquaporin expression in cerebral microvessels : its pathophysiological significance
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