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Identification and analysis of human spermatogonial stem cells

Identification and analysis of human spermatogonial stem cells
人精原干细胞的鉴定与分析
批准号:
20791127
负责人:
KOBAYASHI Hideyuki
金额:
$2.83万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2009

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中文摘要
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英文摘要
Isolated cells from testicular tissues were maintained in serum-free medium, selected growth factors, and STO feeder layer for 1 week. We found that magnetic-activated cell sorting (MACS) was an efficient method to obtain Thy-1+ cells from testis cell suspensions. Enriched Thy-1+ cells were present among unretained cells. Thy-1+ cells were expressed human embryonic stem cell genes. Sixteen weeks after transplanting the Thy-1+ cells into nude mice, there was no evidence of teratoma.
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The characterization and function of male germline stem cells obtained from human testicular tissue
从人类睾丸组织中获得的雄性生殖干细胞的特征和功能
DOI: --
发表时间: 2008
期刊:
影响因子: --
作者: [Kobayashi H, Nagao K, Nakajima K, Miura K, Ishii N, Hideyuki Kobayashi, 小林秀行]
通讯作者: 小林秀行
ヒト精巣組織からのヒト精子幹細胞human spermatogonial stem cells(SSCs)の分離及び培養の試み
尝试从人类睾丸组织中分离和培养人类精原干细胞(SSC)
DOI: --
发表时间: 2008
期刊:
影响因子: --
作者: [Kobayashi H, Nagao K, Nakajima K, Miura K, Ishii N, Hideyuki Kobayashi, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行]
通讯作者: 小林秀行
ヒト精巣組織か照のヒト精子幹細胞human spermatogonial stem cells(SSCs)の分離及び培養の試み
尝试从人类睾丸组织中分离和培养人类精原干细胞(SSC)
DOI: --
发表时间: 2008
期刊:
影响因子: --
作者: [Kobayashi H, Nagao K, Nakajima K, Miura K, Ishii N, Hideyuki Kobayashi, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行, 小林秀行]
通讯作者: 小林秀行
ヒト精巣組織からのヒト精子幹細胞human spermatogonial stem cells(SSCs)の分離の試み及び機能解析
从人类睾丸组织中分离人类精原干细胞(SSC)的尝试和功能分析
DOI: --
发表时间: 2008
期刊:
影响因子: --
作者: [Kobayashi H, Nagao K, Nakajima K, Miura K, Ishii N, Hideyuki Kobayashi, 小林秀行, 小林秀行, 小林秀行, 小林秀行]
通讯作者: 小林秀行
7
    Elucidation of male infertility by epigenome analysis of iPS cells derived from male infertility and stem cell research
    • 批准号:
      16K11072
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.0万
    • 财政年份:
      2016
    • 负责人:
      KOBAYASHI Hideyuki
    • 依托单位:
    The breakthrough of male infertily by stem cell research
    • 批准号:
      24791674
    • 项目类别:
      Grant-in-Aid for Young Scientists (B)
    • 资助金额:
      $2.75万
    • 财政年份:
      2012
    • 负责人:
      KOBAYASHI Hideyuki
    • 依托单位:
    Educational Visual Acuity Assessment of Children with Profound and Multiple Disabilities
    • 批准号:
      22531070
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $1.58万
    • 财政年份:
      2010
    • 负责人:
      KOBAYASHI Hideyuki
    • 依托单位:
    Research of the business dispute resolution process from a viewpointof legal negotiation
    • 批准号:
      22530076
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.91万
    • 财政年份:
      2010
    • 负责人:
      KOBAYASHI Hideyuki
    • 依托单位:
    海外基金