Identification and analysis of human spermatogonial stem cells

睾丸组织分离细胞在无血清培养基、选定的生长因子和STO饲养层中维持1周。我们发现磁活化细胞分选（MACS）是一种从睾丸细胞悬浮液中获得Thy-1+细胞的有效方法。未保留细胞中存在富集的Thy-1+细胞。它们-1+细胞表达人胚胎干细胞基因。将Thy-1+细胞移植到裸鼠体内16周后，未发现畸胎瘤的迹象。

Isolated cells from testicular tissues were maintained in serum-free medium, selected growth factors, and STO feeder layer for 1 week. We found that magnetic-activated cell sorting (MACS) was an efficient method to obtain Thy-1+ cells from testis cell suspensions. Enriched Thy-1+ cells were present among unretained cells. Thy-1+ cells were expressed human embryonic stem cell genes. Sixteen weeks after transplanting the Thy-1+ cells into nude mice, there was no evidence of teratoma.