Analysis for control systems of heme oxygenase-1 in human villus cells and development for immunohistochemical staining of heme oxygenase-1 in human placenta

人绒毛细胞中血红素加氧酶-1控制系统的分析及人胎盘中血红素加氧酶-1免疫组织化学染色的开发

• 批准号: 20791160
• 负责人: SUZUKI Hirotada
• 金额: $ 2.41万
• 依托单位: Jichi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791160/
• 关键词: 産科学; 医療・福祉; 妊娠高血圧腎症; sFlt-1; sEndoglin; HO-1

We cultured primary human trophoblast or BeWo cell with ZuPP (heme oxygenase-1 inhibitor) and analized the mRNA levels of soluble fms-like tyrosine kinase-1 (sFlt-1) and soluble endoglin (sEng) by RT-RCR. These mRNA levels did not change.We are now collecting human placenta tissues. In future, we will try immunohistochemical staining of heme oxygenase-1 in these placenta tissues.

用ZUPP(血红素加氧酶-1抑制剂)培养人滋养层细胞或BeWo细胞，用RT-RCR法检测sFMS样酪氨酸激酶-1(sFlt-1)和sEnoglin(SEnoglin)的mRNA水平。这些信使核糖核酸水平没有变化。我们现在正在收集人类胎盘组织。在未来，我们将尝试在这些胎盘组织中进行血红素加氧酶-1的免疫组织化学染色。