Down-regulation of salivary glands AQP5 by LPS via NF-κB signaling

我们的结果表明，内毒素诱导的AQP5表达的潜在下调是通过这两类转录因子-NF-K13和p-c-jun/c-Fos的复合体介导的。AQP5启动子上的两个核因子-κB结合位点在脂多糖诱导的下调中起着非常重要的作用。免疫共沉淀分析表明，这些转录因子在脂多糖处理后的RAW264.7细胞中有物理结合。脂多糖下调AQP5的机制将在未来利用c-Fos稳定过表达的细胞株来确定。

Our results indicated that LPS induced potential down-regulation of expression of AQP5 is mediated via a complex (es) of these 2 classes of transcription factors, NF- K13 and p-c-jun/c-Fos. The two NF-κB binding sites in the AQP5 promoter played very important roles in the LPS-induced down-regulation. By co-immunoprecipitation analysis, the physical association of these transcription factors was shown in RAW264.7 cell line after LPS treatment. The mechanism of the down-regulation of AQP5 by LPS will be identified using c-Fos stable overexpression cell lines in the future.