Basic research of molecular targeting therapy targeted to SP cells in oral cancer

SP细胞分子靶向治疗口腔癌的基础研究

• 批准号: 20791534
• 负责人: YANAMOTO Souichi
• 金额: $ 2.66万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791534/
• 关键词: 口腔癌; 癌幹細胞; SP細胞

To determine whether human oral cancer cell lines contain a SP cell fraction, we performed flow cytometry analysis and sorting, followed by culturing in serum-free medium (SFM) using the SCC25 tongue cancer cell line. SCC25 cells contained 0.23% SP cells. The fraction of SP cells was available to grow in SFM cultures. SP cells showed higher mRNA expression of stem cell markers (ABCG2, Oct-4 and EpCAM) as compared with non-SP cells. Moreover, SP cells demonstrated more drug resistance to 5-FU, as compared with non-SP cells. The clone formation efficiency of SP cells was significantly higher than non-SP cells at an equal cell number.

为了确定人口腔癌细胞系是否含有SP细胞部分，我们进行了流式细胞术分析和分选，然后使用SCC 25舌癌细胞系在无血清培养基（SFM）中培养。SCC-25细胞含0.23%的SP细胞。SP细胞的部分可用于在SFM培养物中生长。与非SP细胞相比，SP细胞显示出更高的干细胞标志物（ABCG 2、Oct-4和EpCAM）的mRNA表达。此外，与非SP细胞相比，SP细胞对5-FU表现出更高的耐药性。在相同细胞数下，SP细胞的克隆形成效率显著高于非SP细胞。

项目成果

Overexpression of metastasis-associated MTA1 in oral squamous cell carcinomas: correlation with metastasis and invasion

• DOI: 10.1016/j.ijom.2008.05.020
• 发表时间: 2008-11-01
• 期刊: INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
• 影响因子: 2.4
• 作者: Kawasaki, G.;Yanamoto, S.;Mizuno, A.
• 通讯作者: Mizuno, A.

Characteristics of cancer stem like side population cell in oral squamous cell carcinoma

口腔鳞状细胞癌中癌干样侧群细胞的特征

• 发表时间: 2009
• 作者: 柳本惣市;川崎五郎;山田慎一;吉冨泉;水野明夫;Souichi Yanamoto
• 通讯作者: Souichi Yanamoto

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RNAi-mediated down-regulation of alpha-actinin-4 decreases invasion potential in oral squamous cell carcinoma

RNAi介导的α-actinin-4下调降低口腔鳞状细胞癌的侵袭潜力

• 发表时间: 2010
• 期刊: Int J Oral Maxillofac Surg
• 作者: Yamada S;Yanamoto S;Yoshida H;Yoshitomi I;Kawasaki G;Mizuno A;Nemoto TK
• 通讯作者: Nemoto TK

Ribonucleotide reductase small subunit p53R2 promotes oral cancer invasion via the E-cadherin/β-catenin pathway

• DOI: 10.1016/j.oraloncology.2008.07.005
• 发表时间: 2009-06-01
• 期刊: ORAL ONCOLOGY
• 影响因子: 4.8
• 作者: Yanamoto, Souichi;Kawasaki, Goro;Mizuno, Akio
• 通讯作者: Mizuno, Akio