Basic research of molecular targeting therapy targeted to SP cells in oral cancer

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To determine whether human oral cancer cell lines contain a SP cell fraction, we performed flow cytometry analysis and sorting, followed by culturing in serum-free medium (SFM) using the SCC25 tongue cancer cell line. SCC25 cells contained 0.23% SP cells. The fraction of SP cells was available to grow in SFM cultures. SP cells showed higher mRNA expression of stem cell markers (ABCG2, Oct-4 and EpCAM) as compared with non-SP cells. Moreover, SP cells demonstrated more drug resistance to 5-FU, as compared with non-SP cells. The clone formation efficiency of SP cells was significantly higher than non-SP cells at an equal cell number.