Molecular targeting therapy targeted to EpCAM as cancer stem cell marker in oral cancer

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I focused microRNA-21 (miR-21) targeting EpCAM as a molecularrelated tumor invasion. To determine the miR-21 target, I searched for molecular gene that involved in tumor invasion by using commonly cited prediction program miRanda. In oral tongue squamous cell carcinoma (OTSCC) cell line, SCC25 cells, I further evaluated whether miR-21 contributes to cell invasiveness by blocking its expression with a specific knock down LNA probe and validated direct target by Matrigel invasion assay and Western blot. I selected DKK2, as a Wnt/antagonist that involved in tumor invasion. miR-21 overexpression was significantly correlated with DKK2-/β-catenin- immunohistochemical phenotype. Knock down of miR-21 significantly decreases the invasion potential of SCC25 cells with up-regulated DKK2. These results suggest that miR-21 may provide potential therapeutic target for OTSCC treatment.