Studies on the immune-response of γδT cells in animal model with osteoporosis taking bisphosphonates.

服用双磷酸盐的骨质疏松动物模型中γδT细胞免疫反应的研究。

• 批准号: 20791543
• 负责人: ABE Takahiro
• 金额: $ 2.41万
• 依托单位: Saitama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791543/
• 关键词: ゾレドロン酸; コルチコステロイド; RANKL; OPG; TCR; ゾレドロネート; osteoprotegerin; αβTCR; γδTCR

The aim of this study was to examine, in a basic setting, changes in receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG), and the expression of T cell receptor (TCR) in mice treated with zoledronate (ZOL) and prednisolone (PSL) and lipopolysaccharide (LPS). C57BL/6, NOD-scid, and NOD-scid transplanted peripheral blood mononuclear cells (PBMC) were used. Peripheral blood samples were obtained to assay for OPG and RANKL. Femur and mandible were prepared for histomorphometric analysis. The microarchitecture of the bone samples was assessed by micro-CT. TCR expression was analyzed by immunohistochemistry. Histomorphometric analysis indicated that OS/BS and OV/BV were lowered by the administration of ZOL. Ob.S/BS and Oc.S/BS were decreased. In the C57BL/6 group taking both of ZOL and PSL, injection of LPS into the oral mucosa increased the levels of serum-RANKL. We showed that combined administration of ZOL with PSL decreased bone turn-over, and furthermore the combination causes a different pattern of TCR expression. These findings suggest that PSL and LPS have differential effects on bone metabolism and the immunoresponse to ZOL.

本研究的目的是研究在基本环境下，用唑来膦酸钠（ZOL）、强的松龙（PSL）和脂多糖（LPS）处理小鼠NF-kB配体受体激活剂（RANKL）和骨保护素（OPG）的变化，以及T细胞受体（TCR）的表达。采用C57BL/6、NOD-scid和NOD-scid移植外周血单个核细胞（PBMC）。取外周血进行OPG和RANKL测定。制备股骨和下颌骨进行组织形态学分析。采用micro-CT对骨样品进行显微结构分析。免疫组化分析TCR表达。组织形态学分析表明，ZOL降低了OS/BS和OV/BV。b. s /BS和c。S/BS降低。在同时服用ZOL和PSL的C57BL/6组中，口腔黏膜注射LPS使血清rankl水平升高。我们发现ZOL与PSL联合使用可降低骨周转率，并且联合使用可引起不同的TCR表达模式。这些结果表明，PSL和LPS对骨代谢和ZOL免疫应答有不同的影响。

项目成果

骨破壊を呈した顎放線菌症1例

颌骨放线菌病伴骨质破坏1例

• 发表时间: 2008
• 期刊: 日本口腔診断学会誌 21(1)
• 作者: 安部貴大;今井謙一郎;坂田康彰;中本紀道;小林明男;依田哲也
• 通讯作者: 依田哲也

Acetylcholineは骨芽細胞の分化を抑制し増殖を促進する

乙酰胆碱抑制成骨细胞分化并促进增殖

• 发表时间: 2008
• 作者: 佐藤毅;安部貴大;中本紀道;古敷谷昇;福島洋介;古株彰一郎;坂田康彰;都丸泰寿;小林明男;依田哲也
• 通讯作者: 依田哲也

TS-1が著効を示した高齢者下咽頭進行癌の2例

TS-1有效治疗老年晚期下咽癌2例

• 发表时间: 2009
• 期刊: 第22回関越DIF研究会講演集(癌と化学療法社)
• 作者: 高城文彦;菅澤正;松村聡子;盛田恵;嶋村由美子;安部貴大;中平光彦;肥後隆三郎
• 通讯作者: 肥後隆三郎

A randomized controlled trial assessing the effectiveness of professional oral care by dental hyginists

一项随机对照试验，评估牙科保健员专业口腔护理的有效性

• 发表时间: 2008
• 期刊: Int J Dent Hyg 6
• 作者: Sato T;Abe T;Ichikawa M;Fukushima Y;Nakamoto N;Koshikiya N;Kobayashi A;Yoda T
• 通讯作者: Yoda T

BMPの骨芽細胞分化誘導作用におけるSmad1およびSmad4依存的シグナルの解析

BMP 成骨细胞分化诱导作用中 Smad1 和 Smad4 依赖性信号的分析

• 发表时间: 2008
• 作者: 安部貴大;堀直子;佐藤毅;嶋村由美子;小林明男;依田哲也;安部貴大;野島淳也
• 通讯作者: 野島淳也