Analysis of the mechanism of Calcium-sensing receptor activation in cementoblasts

成牙骨质细胞钙敏感受体激活机制分析

• 批准号: 20791605
• 负责人: KANAYA Sousuke
• 金额: $ 2.5万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791605/
• 关键词: セメント芽細胞; 細胞外カルシウム; FGF-2; Calcium-sensing receptor; 細胞外Ca^<2+>

We found that elevated levels of extracellular Ca^<2+> increase fibroblast growth factor (FGF)-2 gene expression in cementoblasts. Pretreatment with a protein kinase A (PKA) inhibitor, H89, or an adenylate cyclase inhibitor, MDL-12330A, inhibited Ca^<2+>-stimulated Fgf-2 expression. In contrast, pretreatment with the protein kinase C (PKC) inhibitor GF-109203X or the phospholipase C (PLC) inhibitor U73122 did not affect the expression of Fgf-2 transcripts, suggesting that the increase in Fgf-2 expression was dependent on the PKA but not the PLC/PKC signaling pathway.

我们发现细胞外Ca 2+ 水平升高增加成牙骨质细胞中成纤维细胞生长因子(FGF)-2基因表达。用蛋白激酶A(PKA)抑制剂H89或腺苷酸环化酶抑制剂MDL-12330A预处理抑制Ca 2+ 刺激的Fgf-2表达。相反，用蛋白激酶C（PKC）抑制剂GF-109203X或磷脂酶C（PLC）抑制剂U73122预处理并不影响Fgf-2转录物的表达，表明Fgf-2表达的增加依赖于PKA而不是PLC/PKC信号通路。

项目成果

Porphyromonas gingivalis fimbriae induce unique dendritic cell subsets via Toll-like receptor 2

• DOI: 10.1111/j.1600-0765.2008.01149.x
• 发表时间: 2009-08-01
• 期刊: JOURNAL OF PERIODONTAL RESEARCH
• 影响因子: 3.5
• 作者: Kanaya, S.;Nemoto, E.;Shimauchi, H.
• 通讯作者: Shimauchi, H.

Elevated extracellular calcium increases expression of bone morphogenetic protein-2 gene via a calcium channel and ERK pathway in human dental pulp cells

• DOI: 10.1016/j.bbrc.2010.03.135
• 发表时间: 2010-04-16
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Tada, Hiroyuki;Nemoto, Eiji;Shimauchi, Hidetoshi
• 通讯作者: Shimauchi, Hidetoshi

Elevated extracellular Ca2+ induces fibroblast growth factor-2 mRNA in cementoblasts

细胞外 Ca2+ 升高诱导成牙骨质细胞中成纤维细胞生长因子 2 mRNA

• 发表时间: 2008
• 作者: S. Kanaya;E. Nemoto;M. Somerman;H. Shimauchi
• 通讯作者: H. Shimauchi

Wnt signaling inhibits cementoblast differentiation and promotes proliferation

• DOI: 10.1016/j.bone.2008.12.029
• 发表时间: 2009-05-01
• 期刊: BONE
• 影响因子: 4.1
• 作者: Nemoto, Eiji;Koshikawa, Yohei;Shimauchi, Hidetoshi
• 通讯作者: Shimauchi, Hidetoshi

マウスセメント芽細胞における細胞外Ca2+刺激によるFGF-2発現誘導

细胞外 Ca2+ 刺激诱导小鼠成牙骨质细胞表达 FGF-2

• 发表时间: 2008
• 作者: 金谷聡介;根本英二;島内英俊
• 通讯作者: 島内英俊