Study on the action mechanism of inhibition of natural origin on lipid droplet formation in macrophages.

研究了真菌beauverolide对巨噬细胞脂滴形成的作用机制。生化研究表明，beauverolide抑制小鼠巨噬细胞酰基辅酶a：胆固醇酰基转移酶（ACAT）。此外，该化合物选择性地抑制完整细胞中的ACAT1同工酶，同时抑制微粒体中的ACAT1和acat2同工酶。细胞生物学研究表明，完整细胞和微粒体之间的差异可能是由于两种同工酶之间活性位点的膜拓扑结构不同所致。

The action mechanism of fungal beauveriolide on lipid droplet formation in macrophages was studied. Biochemical study revealed that beauveriolide inhibited acyl-CoA:cholesterol acyltransferase (ACAT) in mouse macrophages. Furthermore, the compound selectively inhibited ACAT1 isozyme in intact cells, while it inhibited both ACAT1 andACAT2 isozymes in microsomes. Cell biological study suggested that this discrepancy between intact cells and microsome might be due to the different membrane topology of the active sites between the two isozemes.