Pericyte coverage of differentiated microvessels in clear cell renal cell carcinoma correlates with poorer prognosis

目的：以周细胞为标志的血管成熟的临床意义尚不清楚。在本研究中，我们评估血管成熟和患者的预后之间的相关性在CCRCC.Experimental Design：我们分析了福尔马林固定，石蜡包埋组织切片从162原发性和20转移性CCRCC患者。用周细胞标记物抗SMA抗体（作为血管成熟的指标）和分化的血管标记物抗CD34抗体对切片进行双重染色。Nuance多光谱成像系统用于定量这两种细胞类型。我们研究了周细胞覆盖率（PC）和临床病理参数，如肿瘤分级，转移状态，和患者survival.Results：低PC与肿瘤分级和患者的预后差显着相关。低PC也与转移显著相关。分化血管越成熟，预后越好。本研究表明，血管成熟在CCRCC中是重要的。

Purpose : The clinical significance of vascular maturation, which is marked by the presence of the pericytes, is still unclear. In the present study, we evaluated the correlation between vascular maturation and patients' prognosis in CCRCC.Experimental Design : We analyzed formalin-fixed, paraffin-embedded tissue sections from 162 primary and 20 metastatic CCRCC patients. The sections were double-stained with pericyte marker anti-SMA antibody as an indicator of vascular maturation and the differentiated blood vessel marker anti-CD34 antibody. The Nuance Multispectral Imaging System was used to quantify those two cell types. We investigated the relationship between pericyte coverage(PC) and the clinicopathological parameters such as tumor grades, metastasis status, and patient survival.Results : Lower PC were significantly correlated with higher tumor grades and poorer patient's prognosis. Lower PC was also significantly correlated with metastasis. More matured differentiated blood vessels seem to indicate better prognosis. The present study suggested that vascular maturation in CCRCC was important.