DEVELOPMENT OF THE INFRARED SPECTROSCOPIC METHOD USING INTEGRATING SPHERE OPTICS FOR THE NONINVASIVE QUANTITATIVE EVALUATION OF IMPLANTED CELLS

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Objective of this study is the development of a system can which can measure the optical properties of cells in an infrared wavelength range. In this study, we developed the infrared spectroscopic system with double integrating spheres optics for mid-infrared wavelength region and the sample holder which can determine an accurate thickness of the sample. By using the developed system, the all transmittance spectrum of a mesenchymal stem cell line derived from mouse bone marrow, Kusa-A1, was measured. This technique may achieve to get effective spectroscopic information about cell differentiation and so on, quantitatively.