Proteomic analysis of transgenic rat specifically expressing ADAMTS-8 in podocyte

足细胞特异性表达 ADAMTS-8 的转基因大鼠的蛋白质组学分析

• 批准号: 20590951
• 负责人: GOTO Shin
• 金额: $ 2.91万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590951/
• 关键词: ADAMTS-8; transgenic rat; proteome; nephrotic syndrome; phage display; proteinuria; immunoprecipitation

Nephrotic syndrome present massive proteinuria, however, underling mechanisms remain elusive. We focused an interaction between the cell-cell contact which is involved with urine production, and proteinase produced in these cells (ADAMTS-8). Rat with forced expression of ADAMTS-8 gradually showed proteinuria. Using these rat model, we revealed molecules associated with development of proteinuria.

肾病综合征表现为大量的蛋白尿，然而，潜在的机制仍然难以捉摸。我们重点研究了与尿液产生有关的细胞-细胞接触与这些细胞产生的蛋白水解酶之间的相互作用(ADAMTS-8)。强迫表达ADAMTS-8的大鼠逐渐出现蛋白尿。利用这些大鼠模型，我们揭示了与蛋白尿发展相关的分子。

项目成果

Aberrant glycosylation of IgAl is heritable in familial IgA nephropathy.

IgA 异常糖基化在家族性 IgA 肾病中具有遗传性。

• 发表时间: 2008
• 作者: Wada M;Goto S;et al.
• 通讯作者: et al.

Proteomics database in chronic kidney disease.

• DOI: 10.1053/j.ackd.2010.09.005
• 发表时间: 2010-11
• 期刊: Advances in chronic kidney disease
• 影响因子: 2.9
• 作者: Tadashi Yamamoto

Clinicopathological characteristics of patients with IgG4-related tubulointerstitial nephritis

• DOI: 10.1038/ki.2010.271
• 发表时间: 2010-11-01
• 期刊: KIDNEY INTERNATIONAL
• 影响因子: 19.6
• 作者: Saeki, Takako;Nishi, Shinichi;Narita, Ichiei
• 通讯作者: Narita, Ichiei

Aberrant glycosylation of IgA1 is heritable in familial IgA nephropathy.

IgA1 异常糖基化在家族性 IgA 肾病中具有遗传性。

• 发表时间: 2008
• 作者: Wada M;Goto S;et al.
• 通讯作者: et al.

Uremic pruritus in chronic hemodialysis patients.

• 发表时间: 2008-03
• 期刊: Journal of nephrology
• 影响因子: 3.4
• 作者: I. Narita;S. Iguchi;K. Omori;F. Gejyo