AQP1 overexpression in the capillary endothelial cells for the enhancement of muscle regeneration and its therapeutic application to myopathies

AQP1 在毛细血管内皮细胞中过表达以增强肌肉再生及其在肌病中的治疗应用

• 批准号: 20591030
• 负责人: WAKAYAMA Yoshihiro
• 金额: $ 2.91万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591030/
• 关键词: 臨床神経形態学; AQP1; 骨格筋; 再生; 血管内皮; 治療; AQP1 Tgマウス; アクアポリン1

Adenovirus vector with AQP1 (experimental group : EG) or that with Lac Z (control group : CG) was injected into the skeletal muscle necrotic tissue of normal or mdx mouse hind limb, respectively. The necrotic and regenerative skeletal muscle tissues after 3 days of injection showed that numerous blood vessels with anti-AQP1 antibody immunopositive staining were present, although their numbers appeared not to be different between EG and CG. The AQP1 mRNA levels of muscle regenerative tissues after 3 days of injection were not statistically different between two groups both in normal and mdx mice. Furthermore, AQP1 overexpressing transgenic mouse is now under generation by using LNL-AQP1 expression system. The DNA fragments were injected into the ova of 188 mice and finally we obtained one male mouse with transgene.

将含有AQP1的腺病毒载体（实验组：EG）和含有Lac Z的腺病毒载体（对照组：CG）分别注射到正常小鼠和mdx小鼠后肢骨骼肌坏死组织中。注射3天后的坏死和再生骨骼肌组织显示有大量抗aqp1抗体免疫阳性染色的血管存在，尽管它们的数量在EG和CG之间没有差异。注射3 d后，两组正常小鼠和mdx小鼠肌肉再生组织AQP1 mRNA水平均无统计学差异。此外，利用LNL-AQP1表达系统，目前正在培育AQP1过表达转基因小鼠。将这些DNA片段注射到188只小鼠的卵子中，最终获得了一只转基因雄性小鼠。