AQP1 overexpression in the capillary endothelial cells for the enhancement of muscle regeneration and its therapeutic application to myopathies

点击翻译按钮获取中文摘要

Adenovirus vector with AQP1 (experimental group : EG) or that with Lac Z (control group : CG) was injected into the skeletal muscle necrotic tissue of normal or mdx mouse hind limb, respectively. The necrotic and regenerative skeletal muscle tissues after 3 days of injection showed that numerous blood vessels with anti-AQP1 antibody immunopositive staining were present, although their numbers appeared not to be different between EG and CG. The AQP1 mRNA levels of muscle regenerative tissues after 3 days of injection were not statistically different between two groups both in normal and mdx mice. Furthermore, AQP1 overexpressing transgenic mouse is now under generation by using LNL-AQP1 expression system. The DNA fragments were injected into the ova of 188 mice and finally we obtained one male mouse with transgene.