Analysis of candidate gene deficient mice cause insulin resistance in Spontaneously Hypertensive Rats

候选基因缺陷小鼠导致自发性高血压大鼠胰岛素抵抗的分析

• 批准号: 20591049
• 负责人: IIZUKA Yoko
• 金额: $ 3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591049/
• 关键词: エネルギー; 糖質代謝異常; 高血圧自然発症ラット(SHR); 原因候補遺伝子; kynurenine aminotransferase 1(KAT-1); 高血圧; インスリン抵抗性; 交感神経活動亢進; ノックアウトマウス; 肥満; 高血圧自然発症ラット; kynurenine aminotransferase 1; 交感神経亢進; 欠損マウス; RNAiアデノウイルスベクター; 高血圧自然発症ラットSHR; ynurenine aminotransferase 1 /

1) Normal delivery, normal growth, and null expression of mRNA and protein in each organization of KAT-1 knockout mouse have been confirmed. KAT-1 knockout mouse demonstrates 2) significant increase of blood pressure under the normal chow, more increase of blood pressure under 12 weeks high salt diet by tail-cuff and telemetry system, 3) the rise of 24-hour urine catecholamine secretion under high salt diet, 4) significant increase of fast blood glucose, the rise of insulin resistance by insulin tolerance test under normal chow, 5) the decrease of the fat pat weight under high fat diet accompanied with increase in activity level.

1)已证实KAT-1基因敲除小鼠的正常分娩、正常生长以及各组织中mRNA和蛋白质的零表达。KAT-1基因敲除小鼠2）正常饮食下血压显著升高，尾袖和遥测系统显示高盐饮食12周血压升高更明显，3）高盐饮食下24小时尿儿茶酚胺分泌增加，4）空腹血糖显著升高，正常饮食下胰岛素耐受试验显示胰岛素抵抗升高，（5）高脂饮食下脂肪块重量的减少伴随着活动水平的增加。

项目成果

Cyclin-dependent kinase inhibitor, p21WAF1/CIP1, is involved in adipocyte differentiation and hypertrophy, linking to obesity, and insulin resistance

• DOI: 10.1074/jbc.m801824200
• 发表时间: 2008-07-25
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Inoue, Noriyuki;Yahagi, Naoya;Shimano, Hitoshi
• 通讯作者: Shimano, Hitoshi

Establishment of kynurenine aminotransferase 1 ( KAT-1) knockout mice as an animal model of spontaneous hypertension and insulin resistance observedin the spontaneously hypertensive rats

犬尿氨酸转氨酶1（KAT-1）基因敲除小鼠自发性高血压动物模型的建立及自发性高血压大鼠胰岛素抵抗

• 发表时间: 2010
• 作者: 飯塚陽子;矢作直也;et al
• 通讯作者: et al

Hormone-sensitive lipase is involved in hepatic, cholesterol ester hydrolysis.

激素敏感脂肪酶参与肝脏胆固醇酯水解。

• 发表时间: 2008
• 期刊: J Lipid Res 49
• 作者: Sekiya M;et.al.
• 通讯作者: et.al.

SHRの高血圧とインスリン抵抗性原因遺伝子kynurenine aminotransferase 1(KAT-1)欠損マウスの作製と解析

犬尿氨酸转氨酶 1 (KAT-1) 缺陷型小鼠的生成和分析，该基因是 SHR 中导致高血压和胰岛素抵抗的基因

• 发表时间: 2010
• 作者: 飯塚陽子;他
• 通讯作者: 他

Palmitate impairs and eicosapentaenoate restores insulin secretion through regulation of SREBP-1c in pancreatic islets.

• DOI: 10.2337/db06-1806
• 发表时间: 2008-09
• 期刊: Diabetes
• 影响因子: 7.7
• 作者: Kato T;Shimano H;Yamamoto T;Ishikawa M;Kumadaki S;Matsuzaka T;Nakagawa Y;Yahagi N;Nakakuki M;Hasty AH;Takeuchi Y;Kobayashi K;Takahashi A;Yatoh S;Suzuki H;Sone H;Yamada N
• 通讯作者: Yamada N