Preparation of CCP antibodies library in Rheumatoid Arthritis and its clinical application

类风湿性关节炎CCP抗体库的制备及其临床应用

• 批准号: 20591167
• 负责人: KISHI Hiroyuki
• 金额: $ 2.91万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591167/
• 关键词: リウマチ; 自己抗体; 抗CCP抗体; マイクロウェルアレイチップ; ISAAC法; リンパ球チップシステム; 関節リウマチ; CCP; 抗体; エピトープ

In rheumatoid arthritis (RA), appearance of antibodies that bind to cyclic peptide containing citrulline has been reported to be correlated with the onset of the RA and its disease progression. We have hypothesized that the identification of auto-antigens of CCP antibodies might clarify the mechanisms of the induction of CCP antibody production and its pathological function in RA, which may lead to reveal the mechanisms of the progression of joint destruction in RA. To this end, we have developed the ISAAC system that identifies auto-antibody-producing human lymphocytes on lymphocyte-chip. By using ISAAC system, we have obtained auto-antibodies from the peripheral blood of patients of autoimmune diseases, and tried to apply ISAAC system to obtain CCP antibodies from RA patients.

在类风湿性关节炎（RA）中，已报告与含瓜氨酸的环肽结合的抗体的出现与RA的发作及其疾病进展相关。我们推测，CCP抗体自身抗原的鉴定可能有助于阐明RA中CCP抗体产生的诱导机制及其病理作用，从而可能揭示RA关节破坏的进展机制。为此，我们开发了ISAAC系统，在淋巴细胞芯片上识别产生自身抗体的人淋巴细胞。本研究应用ISAAC系统从自身免疫性疾病患者外周血中获得了自身抗体，并尝试应用ISAAC系统从RA患者中获得CCP抗体。

项目成果

ユニバーサルワクチンに向けたHBワクチンの作用機序の検討

检验 HB 疫苗的作用机制，打造通用疫苗

• 发表时间: 2010
• 作者: 田尻和人;ら
• 通讯作者: ら

Analysis of the epitope and neutralizing capacity of human monoclonal antibodies induced by hepatitis B vaccine

• DOI: 10.1016/j.antiviral.2010.04.006
• 发表时间: 2010-07-01
• 期刊: ANTIVIRAL RESEARCH
• 影响因子: 7.6
• 作者: Tajiri, Kazuto;Ozawa, Tatsuhiko;Muraguchi, Atsushi
• 通讯作者: Muraguchi, Atsushi

MAC-CCD systemを用いた細胞内カルシウムの時系列解析とヒトモノクローナルインフルェンザ抗体の単離.

使用 MAC-CCD 系统对细胞内钙进行时间序列分析并分离人单克隆流感抗体。

• 发表时间: 2008
• 作者: 小澤 龍彦;他
• 通讯作者: 他

Enhancement of TRAIL-induced apoptosis with novel TRAIL-Receptor 1 specific human mAbs generated by Immunospot array assay on a chip.

通过芯片上的免疫斑点阵列测定产生的新型 TRAIL 受体 1 特异性人单克隆抗体增强 TRAIL 诱导的细胞凋亡。

• 发表时间: 2011
• 作者: Jin A;Kobayashi E;Ozawa T;Tajiri K;Kishi H;Muraguchi A
• 通讯作者: Muraguchi A

Characterization of human anti-influenza M2 antibody obtained from peripheral blood of vaccinated volunteer.

从接种疫苗的志愿者外周血中获得的人抗流感 M2 抗体的表征。

• 发表时间: 2011
• 作者: Muraguchi A;Ozawa T;Jin A;Tajiri K;Takemoto M;Okuda T;Siraki K;Kishi H.
• 通讯作者: Kishi H.