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The structure and function of beta TCR on immature thymocytes.

β TCR 对未成熟胸腺细胞的结构和功能。

基本信息

批准号：
03807028
负责人：
KISHI Hiroyuki
金额：
$ 1.15万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03807028/
关键词：
betaTCR comples immature thymocytes GPI CD3複合体

项目摘要

Recently it has been shown that the expression of betaTCR is an important step in the differentiation of CD4^-8^- thymocytes into CD4^+8^+ cells ant that the betaTCR chain is expressed on immature thymocytes in the absence of other TCR chains.In order to study the structure of the immature betaTCR complex and its function in thymocyte differentiation,a thymic lymphoma cell line (LSB11-1)was established.LSB11-1 cells express betaTCR on the cell surface but few CD3.Also in this cell line,betaTCR is not associated with alphaTCR.alphaTCR-gene-transfectant of LSB11-1 cells,LSB11-1alpha29, express alphabetaTCR with CD3 complex on the cell surface.Recently it has been shown that IgD molecules could be expressed on the cell surface in the absence of mbl molecules which correspond to CD3 in B cells,and that those IgD molecules are linked with glycosylphosphatidylinositol(GPI). Treatment of LSB11-1 cells with phosphatidylinositol specific phospholipase C(PI-PLC)reduced the expression of betaTCR on LSB11-1 cells,indicating that the betaTCR is GPI-linked,although the expression of alphabetaTCR on LSB11-1alpha29 cells was not changed by PI-PLC treatment.Cell surface expression of GPI-linked betaTCR was also found on CD3^-,betaTCR^+ thymocytes in betaTCR transgenic mice.However CD3^<high>,betaTCR^<high> mature thymocytes did not express GPI-linked betaTCR on the cell surface,indicating that immature thymocytes can express the GPI-linked betaTCR.Although a transient increase in cytoplasmic CA^<2+> was observed when LSB11-1alpha29 cells were stimulated with betaTCR antibodies or CD3 antibodies,cytoplasmic CA^<2+> increase in LSB11-1 cells was not found when stimulated with those antibodies.Presently we are investigating what kind of signal is transduced via the immature betaTCR complex.These results should show the role of immature betaTCR complex in differentiation of immature thymocytes.

为了研究未成熟胸腺细胞βTCR复合体的结构及其在胸腺细胞分化中的作用，建立了胸腺淋巴瘤细胞系(LSB11-1)。该细胞表面表达βTCR，但CD3很少。在该细胞系中，βTCR与αTCR无关。近年来的研究表明，在B细胞中，在缺乏与CD3相对应的MBL分子的情况下，可以在细胞表面表达IGD分子，并且这些IGD分子与糖基磷脂酰肌醇(GPI)相连。用磷脂酰肌醇特异性磷脂酶C(PI-PLC)处理LSB11-1细胞，降低了LSB11-1细胞表面βTCR的表达，表明βTCR是GPI连接的，而未成熟的胸腺细胞表面GPI连接的βTCR的表达没有改变。在CD3^-、BetaTCR^+胸腺细胞表面也发现了GPI连接的BetaTCR的表达。然而，CD3；高、高、高成熟胸腺细胞表面不表达GPI连接的BetaTCR。当LSB11-1Alpha29细胞被βTCR抗体或CD3抗体刺激时，LSB11-1细胞中出现2+和gt；，而在这些抗体刺激下，LSB11-1细胞胞浆中的CA^&lt；2+和gt；没有增加。目前，我们正在研究未成熟的BetaTCR复合体通过什么样的信号转导。这些结果应该表明未成熟的BetaTCR复合体在未成熟胸腺细胞分化中的作用。