A role of T cell receptor beta on immature thymoctye differentiation

T细胞受体β对未成熟胸腺细胞分化的作用

• 批准号: 05670304
• 负责人: KISHI Hiroyuki
• 金额: $ 1.41万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY (1994)Kyushu University (1993)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670304/
• 关键词: Thymus; Cell surface antigen; Differentiation; 未熟胸腺細胞; BT細胞抗原受容体

A monoclonal antibody, 1-23, which recognizes a novel cell surface antigen on immature murine thymocytes (designated as IMT-1 for immature thymocyte antigen-1) was prepared.IMT-1 was found to be expressed on 40-50% of CD4^-8^- (DN), 5-10% of CD4^-8^+ and 5-20% of CD4^++^8 (DP) thymocytes in adult mice, but not expressed on CD4^+8^- thymocytes or peripheral T lymphocytes. In the subset of DN thymocytes, IMT-1 was detected on 40 to 50% of the heat stable antigen^+ (HSA^+), 15% of CD44^+25^+,70% of CD44^-25^+ and 70% of CD44^-25^- populations, whereas it was not detected on HSA^- and CD44^+25^- populations. In fetal thymuses, IMT-1 was expressed on a minor population of thymocytes at day 14.5 and 15.5 of gestation. However, at day 16.5 of gestation, a majority of DN as well as DP thymocytes became IMT-1 positive. These results shows that the expression of IMT-1 is correlative to that of pre-TCR.Addition of the 1-23 antibody to the long-term fetal thymus organ culture reduced the DP thymocyte population. These results indicate the possibility that the IMT-1 may be involved in the regulation of thymocyte differentiation.

制备了一种识别未成熟小鼠胸腺细胞表面新抗原的单抗1-23(IMT-1，即未成熟胸腺细胞抗原-1)，在成年小鼠40-50%的CD4^-8^-(DN)、5-10%的CD4^-8^+(DP)和5-20%的CD4^-8^+(DP)胸腺细胞上表达IMT-1，而在CD4^-8^-胸腺细胞和外周T淋巴细胞上不表达。在糖尿病肾病胸腺细胞亚群中，40%~50%的热稳定抗原^+(HSA^+)、15%的CD44^+25^+、70%的CD44^-25^+和70%的CD44^-25^-人群检测到IMT-1，而在HSA^-和CD44^+25^-人群中未检测到IMT-1。在胎儿胸腺中，IMT-1在妊娠14.5天和15.5天的一小部分胸腺细胞上表达。但在妊娠16.5天，大多数糖尿病肾病和糖尿病胸腺细胞均呈IMT-1阳性。这些结果表明，IMT-1的表达与TCR前的表达有关。在长期培养的胎儿胸腺器官中加入1-23抗体后，DP胸腺细胞的数量减少。这些结果表明，IMT-1可能参与胸腺细胞分化的调节。

项目成果

Tagoh,H.: "Reciprocal IL-1β gene expression in medial and lateral hypothalamic areas in the SART-stressed mice." Nurosci.Lett.184. 17-20 (1995)

Tagoh, H.：“SART 应激小鼠内侧和外侧下丘脑区域的相互 IL-1β 基因表达。”Nurosci.Lett.184（1995）。

Kishi, H., Su, D.M.and Watanabe, T.: "Cell surface antigen, IMT-1, expressed on thymocytes differentiating from CD4-8-to CD4_+8_+." J.Cell.Biochem.18D. 455 (1994)

Kishi, H.、Su, D.M. 和 Watanabe, T.：“细胞表面抗原，IMT-1，在从 CD4-8- 分化为 CD4_ 8_ 的胸腺细胞上表达。”

Tagoh, H., Nishijo, H., Uwano, T., Kishi, H., Ono, T., and Muraguchi, A.: "Reciprocal lL-1beta gene expression in medial and lateral hypothalamic areas in the SART-stressed mice." Neurosci.Lett.184. 17-20 (1995)

Tagoh, H.、Nishijo, H.、Uwano, T.、Kishi, H.、Ono, T. 和 Muraguchi, A.：“SART 应激小鼠下丘脑内侧和外侧区域的 IL-1beta 基因表达相互关系

Kishi,H.: "Cell surface antigen,IMT-1,expressed on thymocytes differentiating from CD4^-8^- to CD4^+8^+" J.Cell.Biochem.180. 455- (1994)

Kishi,H.：“细胞表面抗原，IMT-1，在从 CD4^-8^- 分化为 CD4^8^ 的胸腺细胞上表达”J.Cell.Biochem.180。

Kishi,H.: "Cell surface autigen,IMT-1,expressed on thymocytes differentiating from CD4-8-toCD4+8+." J.Cell.Biochem.18D. 455 (1994)

Kishi, H.：“细胞表面抗原，IMT-1，在从 CD4-8 分化为 CD4 8 的胸腺细胞上表达。”