Mechanism of breakdown of blood-brain barrier accompanied with viral encephalopathy

血脑屏障破坏伴病毒性脑病的机制

• 批准号: 20591228
• 负责人: ASAI Kiyofumi
• 金额: $ 3万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591228/
• 关键词: 急性脳症; 血液脳関門; アストロサイト; 血管内皮細胞; サイトカイン

We have tried to establish immortalized mouse brain endothelial cell lines and rat astrocytes cell line for the co-culture system of both cells. Primary cultured mouse brain endothelial cells were transfected with SV40 T-antigen expressing vector and selected by zeocin and blasticidin. Primary cultured rat astrocytes were transfected with same vectors and selected by some procedures. Three colonies from endothelial cells and 10 colonies from astrocytes were obtained, but in all of the immortalized cell lines, expression of SV40 T was not controlled by tetracycline. Thus Tet-repressor expressing vector was re-transfected to the cell lines and finally 3 astrocyte cell lines were obtained in which expression of SV40 T was controlled by tetracycline. Now we are investigating whether the expression of GFAP changes or not by on/off of SV40 T, and trying to establish co-culture system of endothelial cell lines and astrocyte cell lines.

我们尝试建立永生化小鼠脑内皮细胞系和大鼠星形胶质细胞系，以建立两者共培养体系。原代培养的小鼠脑血管内皮细胞经SV 40 T抗原表达载体转染后，用zeocin和blasticidin进行筛选。原代培养的大鼠星形胶质细胞用相同的载体转染，并通过一些程序进行选择。从内皮细胞获得3个集落，从星形胶质细胞获得10个集落，但在所有永生化细胞系中，SV 40 T的表达不受四环素控制。将四环素抑制子表达载体再次转染到细胞系中，最终获得3株四环素调控SV 40 T表达的星形胶质细胞系。本研究旨在探讨SV 40 T的开启/关闭是否改变GFAP的表达，并尝试建立血管内皮细胞系与星形胶质细胞系的共培养体系。

项目成果

Differential inflammatory gene expression in young and middle aged mice during injury-induced hippocampal neurogenesis.

损伤诱导的海马神经发生过程中年轻和中年小鼠炎症基因表达的差异。

• 发表时间: 2009
• 作者: McPherson C.A.;Aoyama M.;Grissom S.F.;Gohlke J.;Harry G.J.
• 通讯作者: Harry G.J.

Region-specific expression of a water nervous system.

水神经系统的区域特异性表达。

• 发表时间: 2008
• 作者: Aoyama;M.;Kakita;H.;Kato;S.;Fujita;M.;Sobue;K.;Asai;K.
• 通讯作者: K.

FK506 inhibition of gliostatin/thymidine phosphorylase production induced by tumor necrosis factor-alpha in rheumatoid fibroblast-like synoviocytes.

FK506 抑制肿瘤坏死因子-α 在类风湿成纤维细胞样滑膜细胞中诱导的胶质抑素/胸苷磷酸化酶的产生。

• 发表时间: 2010
• 期刊: Rheumatol Int in press
• 作者: Yamagami;T.;Waguri-Nagaya;Y.;Ikuta;K.;Aoyama;M.;Asai;K.;Otsuka;T.
• 通讯作者: T.

The ZHFX3/ATBF1 transcription factor transduces ATM signaling to regulate PDGF receptor beta and protect cerebellar neurons from oxidative stress.

ZHFX3/ATBF1 转录因子转导 ATM 信号传导以调节 PDGF 受体 β 并保护小脑神经元免受氧化应激。

• 发表时间: 2010
• 作者: Nan GAO;Tae-sum Kim;Mitsuko Suzuki;Makoto Kawaguchi;Cha-Gyum Jung;Kiyofumi Asai;Yutaka Miura
• 通讯作者: Yutaka Miura

Role of ES Cell-Expressed Ras (ERas) in Tumorigenicity of Gastric Cancer

• DOI: 10.2353/ajpath.2010.091056
• 发表时间: 2010-08-01
• 期刊: AMERICAN JOURNAL OF PATHOLOGY
• 影响因子: 6
• 作者: Kubota, Eiji;Kataoka, Hiromi;Joh, Takashi
• 通讯作者: Joh, Takashi