Regional differences of astrocytic function

本研究的目的是鉴定星形胶质细胞区域异质性的分子。(1)星形胶质细胞神经营养因子表达的区域异质性。取E18胎鼠大脑皮层、海马、嗅球、小脑和脊髓，分别培养星形胶质细胞。分离总RNA并进行北方印迹分析。胶质细胞成熟因子β广泛表达。胶质成熟因子-γ在小脑和脊髓的表达高于其他区域。脂皮质素1在海马、皮质和小脑有高表达。(2)Atras Array分析星形胶质细胞基因表达的区域异质性取E18胎鼠大脑皮层、海马和小脑分别培养星形胶质细胞。分离总RNA并进行Atras Array（Clontech）分析。比较大脑皮层和海马的基因表达，有7个基因在大脑皮层的表达高于海马，4个基因在海马的表达高于大脑皮层。大脑皮层和小脑比较，5个基因在皮层表达量较高，4个基因在小脑表达量较高。RT-PCR检测Id-1、HSP 27和Lipocortin 1的表达。RT-PCR结果与Atlas Array数据一致。

Our aim of this research was to identify the molecules that exhibit the regional heterogeneity of astrocyte.(1) Regional heterogeneity of the expression of neurotrophic factors in astrocyte. Astrocytes were cultured separately from cortex, hippocampus, olfactory bulb, cerebellum and spinal cord of rat embryo (E18). Total RNA was isolated and subjected to northern blot analysis. Glia maturation factor-beta was expressed ubiquitously. Expression of glia maturation factor-gamma was higher in cerebellum and spinal cord than other regions. High expression of lipocortin 1 was detected in hippocampus, cortex and cerebellum.(2) Analysis of regional heterogeneity of the astrocytic gene expression by Atras Array. Astrocytes were cultured separately from cortex, hippocampus and cerebellum of rat embryo (E18). Total RNA was isolated and subjected to analysis of Atras Array (Clontech). When the gene expression of cortex was compare with that of hippocampus, expression of 7 genes was higher in cortex than hippocampus, and 4 genes were higher in hippocampus than cortex. When compare cortex and cerebellum, 5 genes were higher in cortex, and 4 genes were higher in cerebellum. The expressions of Id-1, HSP27 and lipocortin 1 were confirmed by RT-PCR. The results from RT-PCR were consistent with the data from Atlas Array.