Regional differences of astrocytic function

星形胶质细胞功能的区域差异

• 批准号: 10214202
• 负责人: ASAI Kiyofumi
• 金额: $ 18.62万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10214202/
• 关键词: Astrocyte; Regional difference; Neurotrophic Factor; アストロアサイト; 神経機能調節; グリア成長因子; リポコルチン; SAGE; アトスロサイト; ニューロン; シナプス形成; 神経情報伝達; ギャップジャンクション; カルシウムイオン; 細胞内濃度

Our aim of this research was to identify the molecules that exhibit the regional heterogeneity of astrocyte.(1) Regional heterogeneity of the expression of neurotrophic factors in astrocyte. Astrocytes were cultured separately from cortex, hippocampus, olfactory bulb, cerebellum and spinal cord of rat embryo (E18). Total RNA was isolated and subjected to northern blot analysis. Glia maturation factor-beta was expressed ubiquitously. Expression of glia maturation factor-gamma was higher in cerebellum and spinal cord than other regions. High expression of lipocortin 1 was detected in hippocampus, cortex and cerebellum.(2) Analysis of regional heterogeneity of the astrocytic gene expression by Atras Array. Astrocytes were cultured separately from cortex, hippocampus and cerebellum of rat embryo (E18). Total RNA was isolated and subjected to analysis of Atras Array (Clontech). When the gene expression of cortex was compare with that of hippocampus, expression of 7 genes was higher in cortex than hippocampus, and 4 genes were higher in hippocampus than cortex. When compare cortex and cerebellum, 5 genes were higher in cortex, and 4 genes were higher in cerebellum. The expressions of Id-1, HSP27 and lipocortin 1 were confirmed by RT-PCR. The results from RT-PCR were consistent with the data from Atlas Array.

本研究的目的是鉴定星形胶质细胞表现出区域异质性的分子。(1)星形胶质细胞神经营养因子表达的区域异质性。星形胶质细胞与大鼠胚胎（E18）的皮质、海马、嗅球、小脑和脊髓分开培养。分离总RNA并进行RNA印迹分析。神经胶质细胞成熟因子-β 普遍表达。小脑和脊髓中胶质细胞成熟因子-γ的表达高于其他区域。海马、皮质和小脑均检测到脂皮质素1高表达。(2)Atras Array分析星形胶质细胞基因表达的区域异质性。星形胶质细胞与大鼠胚胎（E18）的皮质、海马和小脑分开培养。分离总RNA并进行Atras Array (Clontech)分析。皮层与海马基因表达量比较，有7个基因在皮层表达量高于海马，有4个基因在海马表达量高于皮层。比较皮质和小脑，5个基因在皮质中较高，4个基因在小脑中较高。 RT-PCR证实Id-1、HSP27和脂皮质素1的表达。 RT-PCR结果与Atlas Array数据一致。

项目成果

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Yoneda, K. 等人：“星形胶质细胞中水通道蛋白 4 表达的调节”Mol Brain Res。

Tsuiki, H. et al.: "Cloning of a rat maturation factor-γ (rGMFG) cDNA and expression of its mRNA and protein in rat organs"J. Biochem.. 127. 517-523 (2000)

Tsuiki, H. 等人：“大鼠成熟因子-γ (rGMFG) cDNA 的克隆及其 mRNA 和蛋白质在大鼠器官中的表达”J. Biochem.. 127. 517-523 (2000)

Okada,Y.: "Celiac ganglionotropic invasion of pancreatic cancer cells bearing c-rat proto-oncogene with reference to GDNF." Int.J.Cancer. (in press).

Okada,Y.：“携带 c-大鼠原癌基因的胰腺癌细胞的腹腔神经节细胞侵袭，参考 GDNF。”

Nakanishi K. et al.: "Recurrent subthreshold electrical activities of rat neocortical neurons progress during long-term culture"Neurosci. Lett.. 304. 85-88 (2001)

Nakanishi K. 等人：“长期培养期间大鼠新皮质神经元的反复阈下电活动进展”Neurosci。

Yamamoto, N. et al.: "Differential regulation of aquaporin expression in astrocytes by protein kinase C"Mol. Brain Res.. 95. 110-116 (2001)

Yamamoto, N. 等人：“蛋白激酶 C 对星形胶质细胞中水通道蛋白表达的差异调节”Mol。