The mechanism of development and exacerbation of virus-induced asthma analyzed by a lipid mediator gene knock-out mice

通过脂质介质基因敲除小鼠分析病毒诱发哮喘的发生和恶化机制

• 批准号: 20591267
• 负责人: KATO Masahiko
• 金额: $ 3万
• 依托单位: Gunma Institute of Public Health and Environmental Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591267/
• 关键词: 小児免疫; アレルギー; 膠原病学; 気管支喘息; ウイルス感染; 好酸球; 脂質メディエーター; 遺伝子

To clarify the pathogenesis of exacerbations of asthma due to viral infection, we examined bronchial resistance, peripheral blood and bronchial alveolar fluid (BALF) analyses and multiple types of cytokines/chemokines using an experimental asthma model mice infected with respiratory syncytial virus (RSV). The levels of BALF and tissue eosinophils showed significant increase in ovalbumin (OVA) and OVA/RSV groups compared with controls. MIP-1αin BALF was significantly increased in OVA/RSV groups compared with RSV groups, OVA groups, and control groups. Serum IL-5 in OVA groups and serum IL-17 in OVA/RSV groups were also higher than in controls. These findings suggest that eosinophilic inflammation via MIP-1α may play an important role in acute exacerbations of asthma model induced by RSV.

为了阐明病毒感染导致哮喘加重的发病机制，我们使用呼吸道合胞病毒（RSV）感染的实验性哮喘模型小鼠，检测了支气管阻力、外周血和支气管肺泡液（BALF）分析以及多种类型的细胞因子/趋化因子。与对照组相比，卵清蛋白（OVA）和OVA/RSV组BALF和组织嗜酸性粒细胞水平显著升高。BALF中MIP-1α在OVA/RSV组较RSV组、OVA组和对照组显著升高。OVA组血清IL-5和OVA/RSV组血清IL-17也高于对照组。提示MIP-1α介导的嗜酸性粒细胞炎症反应可能在呼吸道合胞病毒诱导的哮喘急性加重过程中起重要作用。

项目成果

FIP1L1/PDGFRalpha synergizes with SCF to induce systemic mastocytosis in a murine model of chronic eosinophilic leukemia/hypereosinophilic syndrome.

• DOI: 10.1182/blood-2007-11-126268
• 发表时间: 2008-09
• 期刊: Blood
• 影响因子: 20.3
• 作者: Yoshiyuki Yamada;A. Sánchez-Aguilera;E. Brandt;M. McBride;Nabeel J. H. Al-Moamen;F. Finkelman;David A. Williams;J. Cancelas;M. Rothenberg

ライノウイルスおよびRSウイルスによる小児気管支喘息発作時の血清中サイトカイン/ケモカイン産生と好酸球活性化の相違

由鼻病毒和呼吸道合胞病毒引起的儿童支气管哮喘发作期间血清细胞因子/趋化因子产生和嗜酸性粒细胞活化的差异。

• 发表时间: 2010
• 作者: 加藤政彦;山田佳之
• 通讯作者: 山田佳之

Effects of respiratory syncytial virus infection and major basic protein derived from eosinophils in pulmonary alveolar epithelial cells (A549)

• DOI: 10.1042/cbi20100255
• 发表时间: 2011-05-01
• 期刊: CELL BIOLOGY INTERNATIONAL
• 影响因子: 3.9
• 作者: Ishioka, Taisei;Kimura, Hirokazu;Kato, Masahiko
• 通讯作者: Kato, Masahiko

小児科医のノートから.RSウイルス感染症の最近の動向-勤務医の視点から

来自儿科医生的笔记：RSV 感染的最新趋势——从一名在职医生的角度来看。

• 发表时间: 2010
• 期刊: 群馬県小児科医会便り
• 作者: Kawamura T;Takeda K;Kaneda H;Matsumoto H;Hayakawa Y;Raulet DH;Ikarashi Y;Kronenberg M;Yagita H;Kinoshita K;Abo T;Okumura K;Smyth MJ.;加藤政彦
• 通讯作者: 加藤政彦

Differential effects of systemic corticosteroids on serum eosinophil cationic protein and cytokine profile in rhinovirus- and RS virus-induced acute exacerbation of childhood asthma.

全身皮质类固醇对鼻病毒和 RS 病毒诱导的儿童哮喘急性加重血清嗜酸性粒细胞阳离子蛋白和细胞因子谱的不同影响。

• 发表时间: 2011
• 作者: Kato M;Yamada Y;Maruyama K;Hayashi Y
• 通讯作者: Hayashi Y