Development of new objective methods to assess disease activity in pemphigus using monoclonal single-chain- variable-fragment antibodies

为了建立评价天疱疮疾病活动性的新的客观方法，我们用噬菌体展示技术对从一例叶状天疱疮患者中分离的抗桥粒芯糖蛋白(DSG)1自身抗体进行了详细的鉴定。在许多天疱疮患者中发现了与自身抗体结合的桥粒芯糖蛋白的主要表位。天疱疮血清对表位的反应性与疾病活动性有关。这些发现将有助于开发新的客观方法来评估天疱疮患者的疾病活动性。

In order to develop new objective methods to assess the disease activity in pemphigus, we performed the detailed characterization of repertories of monoclonal anti-desmoglein (Dsg) 1 autoantibodies isolated from a pemphigus foliaceus patient by phage display. Major epitopes on desmoglein bound by autoantibodies in many pemphigus patients were uncovered. The reactivity against the epitopes by pemphigus sera correlates with the disease activity. These findings will facilitate the development of new objective methods to assess the disease activity in pemphigus patients.