BMP, Kit and Mitf on melanocytes and melanoma cells

BMP、Kit 和 Mitf 对黑色素细胞和黑色素瘤细胞的作用

• 批准号: 20591356
• 负责人: KAWAKAMI Tamihiro
• 金额: $ 2.91万
• 依托单位: St.Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591356/
• 关键词: メラノサイト; メラノーマ; BMP; Kit; Mitf; Ret; 神経冠細胞; 悪性黒色腫; マウス神経冠培義系; 血管炎; 抗リン脂質抗体; LAMP2; マウス神経冠培養系; アポトーシス

We previously established three distinct cell populations of mouse neural crest (NC) cells, NCCmelb4, NCCmelb4M5 and NCCmelan5. BMP4 is activated and is involved in the regulation of Kit expression on most immature melanocyte precursors. We further investigated the influence of BMP4 in vitro using primary NC cells cultured from wild-type mice. Addition of BMP4 to the medium increased the number of Kit-positive cells compared to untreated controls. We have identified BMP4 as an important factor for prepubertal Kit-negative melanoblasts just prior to entering the Kit-dependent cycle of melanogenesis. Western blotting revealed expression of the Ret protein in NCCmelb4M5 and in Mel-Ret cells, but in contrast, there was no expression of the Ret protein in NCCmelb4 or NCCmelan5 cells. Immunostaining revealed that NCCmelb4M5 and Mel-Ret cells are positive for Ret, but NCCmelb4 and NCCmelan5 cells are negative for Ret. Thus, Ret protein is expressed in most immature melanoblasts, while melanocytes are negative for Ret. Based on these findings, the combined effects of Ret and BMP4 might provide important clues towards understanding the roles and working mechanisms in melanocyte development.

我们先前建立了小鼠神经嵴（NC）细胞的三种不同细胞群，NCCmelb 4、NCCmelb 4 M5和NCCmelan 5。BMP 4被激活并参与大多数未成熟黑素细胞前体上Kit表达的调节。我们使用从野生型小鼠培养的原代NC细胞进一步研究了BMP 4的体外影响。与未处理的对照相比，向培养基中添加BMP 4增加了Kit阳性细胞的数量。我们已经确定BMP 4作为一个重要的因素，青春期前的Kit阴性的黑素母细胞之前，进入黑素生成的Kit依赖性周期。Western印迹显示Ret蛋白在NCCmelb 4 M5和Mel-Ret细胞中表达，但相比之下，NCCmelb 4或NCCmelan 5细胞中没有Ret蛋白的表达。免疫组化显示NCCmelb 4 M5和Mel-Ret细胞对Ret呈阳性反应，而NCCmelb 4和NCCmelan 5细胞对Ret呈阴性反应。因此，Ret蛋白在大多数未成熟的成黑素细胞中表达，而黑素细胞对Ret呈阴性。基于这些发现，Ret和BMP 4的联合作用可能为理解黑素细胞发育中的作用和工作机制提供重要线索。

项目成果

Granuloma annulare-like skin lesions as an initial manifestation in a Japanese patient with adult T-cell leukemia/lymphoma

• DOI: 10.1016/j.jaad.2008.11.014
• 发表时间: 2009-05-01
• 期刊: JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
• 影响因子: 13.8
• 作者: Kawakami, Tamihiro;Kawanabe, Takeshi;Soma, Yoshinao
• 通讯作者: Soma, Yoshinao

BMP-4 upregulates kit expression in mouse melanoblasts prior to the kit-dependent cycle of melanogenesis

• DOI: 10.1038/sj.jid.5701136
• 发表时间: 2008-05-01
• 期刊: JOURNAL OF INVESTIGATIVE DERMATOLOGY
• 影响因子: 6.5
• 作者: Kawakami, Tamihiro;Kimura, Satoko;Soma, Yoshinao
• 通讯作者: Soma, Yoshinao

Antiphosphatidylserine-prothrombin complex antibodies in 3 patients with Behcet disease involving superficial vein thrombophlebitis.

抗磷脂酰丝氨酸-凝血酶原复合物抗体在 3 例伴有浅静脉血栓性静脉炎的白塞病患者中的应用。

• 发表时间: 2009
• 期刊: Arch Dermatol. 145(2)
• 作者: Kawakami T;et al.
• 通讯作者: et al.

Late appearance of an acute graft-vs.-host disease reaction subsequent to a graft-vs.-tumor effect induced by bone marrow transplantation in a

骨髓移植诱导的移植物抗肿瘤效应后出现急性移植物抗宿主病反应

• 发表时间: 2010
• 期刊: Int J Dermatol.
• 作者: Kawakami T;et al.
• 通讯作者: et al.

Vasculitis from the derm atological point of view From 'The Clinic' to 'The Laboratory Bench'.

从皮肤学角度看血管炎从“诊所”到“实验室工作台”。

• 发表时间: 2010
• 作者: Chang CH;et al.;Kawakami T
• 通讯作者: Kawakami T