BMP, Mitf, and RET on melanocytes and melanoma cell

BMP、Mitf 和 RET 对黑色素细胞和黑色素瘤细胞的影响

• 批准号: 23591658
• 负责人: KAWAKAMI Tamihiro
• 金额: $ 3.33万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591658/
• 关键词: メラノサイト; メラノーマ; BMP; Kit; Mitf; Ret; 皮膚色素細胞; メラノブラスト; 活性型ビタミンD3; 白斑; ヒトiPS細胞; 遺伝性対側性色素異常症; BMP4; 尋常性白斑; マウス神経冠細胞; 血管炎

Western blotting revealed expression of the Ret protein in NCCmelb4M5 and in Mel-Ret cells, but in contrast, there was no expression of the Ret protein in NCCmelb4 or NCCmelan5 cells. Immunostaining also revealed that NCCmelb4M5 and Mel-Ret cells are positive for Ret, but NCCmelb4 and NCCmelan5 cells are negative for Ret. Thus, Ret protein is expressed in most immature melanoblasts, while melanocytes are negative for Ret. BMP-4 was added to the medium and incubated for 3 days at varying concentrations. After incubation with 10 ng/ml BMP-4 for 3 days, Ret protein expression was decreased, and disappeared completely when BMP-4 was added at 20 ng/ml. Ret protein expression in NCCmelb4M5 cells showed a dose-dependent decrease in expression elicited by BMP-4. Based on these findings, the combined effects of Ret and BMP-4 might provide important clues towards understanding the roles and working mechanisms in melanocyte development.

Western blotting结果显示，Ret蛋白在NCCmelb4M5和Mel-Ret细胞中表达，而在NCCmelb4和NCCmelan5细胞中未表达。免疫染色还显示，NCCmelb4M5和Mel-Ret细胞Ret表达阳性，而NCCmelb4和NCCmelan5细胞Ret表达阴性。因此，Ret蛋白在大多数未成熟的黑素母细胞中表达，而黑素细胞Ret表达阴性。将BMP-4加入培养液中，以不同浓度孵育3天。10 ng/ml BMP-4孵育3天后，Ret蛋白表达降低，20 ng/ml BMP-4孵育后Ret蛋白表达完全消失。BMP-4诱导的NCCmelb4M5细胞中Ret蛋白表达呈剂量依赖性降低。基于这些发现，Ret和BMP-4的联合作用可能为理解黑素细胞发育中的作用和工作机制提供重要线索。

项目成果

爪囲線維腫の主訴より診断された結節性硬化症の1例

以甲襞纤维瘤为主诉诊断结节性硬化症1例

• 发表时间: 2011
• 期刊: 皮膚科の臨床
• 作者: 島田恵子;木村聡子;川上民裕;相馬良直
• 通讯作者: 相馬良直

活性型ビタミンD3とレチノイン酸のヒト皮膚メラノサイトとヒト皮膚メラノブラストへの効果

活性维生素D3和视黄酸对人皮肤黑素细胞和人皮肤黑素细胞的影响

• 作者: 川上民裕;大串明子;相馬良直;広部知久
• 通讯作者: 広部知久

14トリソミーが見出されたLinear and whorled nevoid hypermelanosisの1例

14号三体性线状、轮状痣色素沉着症一例

• 发表时间: 2012
• 期刊: 皮膚科の臨床
• 作者: 中川恵子;芳賀恒夫;布施恵理;川上民裕;相馬良直
• 通讯作者: 相馬良直

Correlation of Livedo Racemosa, Cutaneous Inflammatory Plaques, and Antiphospholipid Antibodies in Patients With Cutaneous Polyarteritis Nodosa

• DOI: 10.1097/md.0b013e3182115508
• 发表时间: 2011-03-01
• 期刊: MEDICINE
• 影响因子: 1.6
• 作者: Kawakami, Tamihiro;Soma, Yoshinao
• 通讯作者: Soma, Yoshinao

Disease severity indexes and treatment evaluation criteria in vitiligo.

• DOI: 10.1155/2011/750342
• 发表时间: 2011
• 期刊: Dermatology research and practice
• 影响因子: 1.5
• 作者: Kawakami T;Hashimoto T
• 通讯作者: Hashimoto T