Promotion of Liver Regeneration and Control of Infectious Complications after Liver Transplantation and Major Hepatectomy.

促进肝脏再生和控制肝移植和主要肝切除术后的感染并发症。

• 批准号: 20591601
• 负责人: TAKEUCHI Dan
• 金额: $ 3万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591601/
• 关键词: 肝臓外科学; 感染症; 移植・再生医療; 免疫学; 外科; シグナル伝達

In rat model, 90% hepatectomy caused neutrophil dependent liver tissue injury, impaired liver regeneration, resulting in poor survival. This was accompanied by TGF-β production by HSC and suppressed expression of MRP-2 by hepatocytes. Liver tissue injury was attenuated after 70% hepatectomy. Kuppfer cells from remnant liver produced HGF and EGF. Humoral agents produced by cells in sinusoidal space were supposed to be important for liver regeneration.In patients developing infectious complications after hepatectomy, neutrophils produced IL-1β, TNFα, and CCL2 3 days after surgery, and CD14^+ monocytes showed properties of M2 macrophages. In patients developing severe SIRS, preoperative neutrophils produced IL-1β and TNFα.In murine model, administration of steroids caused impaired recruitment of inflammatory cells to the site of viral infection after hepatectomy. This also caused impaired production of α-defensin by neutrophils resulting in prolonged viral infection.

在大鼠模型中，90%的肝切除术引起嗜中性粒细胞依赖性肝组织损伤，肝脏再生受损，导致生存差。这伴随着HSC产生TGF-β和肝细胞抑制MRP-2的表达。肝切除70%后，肝组织损伤减轻。残肝Kuppfer细胞产生HGF和EGF。肝窦腔细胞产生的体液制剂对肝再生有重要作用。在肝切除术后出现感染性并发症的患者中，中性粒细胞在术后3天产生IL-1β、TNFα和CCL2， CD14^+单核细胞表现出M2巨噬细胞的特性。在发生严重SIRS的患者中，术前中性粒细胞产生IL-1β和TNFα。在小鼠模型中，给药类固醇导致肝切除术后炎症细胞募集到病毒感染部位受损。这也导致中性粒细胞α-防御素的产生受损，导致病毒感染时间延长。

项目成果

Excessive inflammation but decreased immunological response renders liver susceptible to infection in bile duct ligated mice

过度炎症但免疫反应降低导致胆管结扎小鼠肝脏易受感染

• 发表时间: 2008
• 期刊: J Surg Res 246
• 作者: Morita Y;Yoshidome H;Kimura F;Takeuchi D;Miyazaki M;et.al.
• 通讯作者: et.al.

Cooperative Role of Macrophages and Neutrophils in Host Antiprotozoan Resistance in Mice Acutely Infected with Cryptosporidium parvum

巨噬细胞和中性粒细胞在急性隐孢子虫感染小鼠宿主抗原虫耐药性中的协同作用

• DOI: 10.1128/iai.00112-08
• 发表时间: 2008
• 期刊: Infection and Immunity
• 影响因子: 3.1
• 作者: D. Takeuchi;V. C. Jones;Makiko Kobayashi;F. Suzuki
• 通讯作者: F. Suzuki

Successful Treatment for Pancreatic Injury Presented with Pancreatic Ascites 2 Months after a Blunt Abdominal Trauma.

腹部钝性外伤 2 个月后出现胰腹水的胰腺损伤的成功治疗。

• 期刊: Hepatogastroenterology (In press)
• 作者: Miyazaki M;Yoshidome H;Ohtsuka M;Kato A;Takeuchi D;et. al.;Morita Y.;Miyazaki M.;Takeuchi D.
• 通讯作者: Takeuchi D.

高度脈管侵襲(Vp3/4・Vv3)を伴う進行肝細胞癌に対する外科治療の意義

晚期肝细胞癌伴晚期血管侵犯（Vp3/4/Vv3）的手术治疗意义

• 发表时间: 2009
• 作者: 竹内男;吉留博之;木村文夫;清水宏明;大塚将之;加藤厚;吉富秀幸;古川勝規;三橋登;宮崎勝
• 通讯作者: 宮崎勝

軽症熱傷により誘導される感染抵抗性についての検討

轻度烧伤所致感染抵抗力的研究

• 发表时间: 2008
• 作者: 竹内男;吉留博之;木村文夫;清水宏明;大塚将之;加藤厚;吉富秀幸;古川勝規;野沢聡志;三橋登;高屋敷吏;須田浩介;高野重紹;宮崎勝
• 通讯作者: 宮崎勝