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Regulation of organ dysfunction and infectious complications after liver transplantation : in the view of innate immunity and the Kupffer cells

肝移植后器官功能障碍和感染并发症的调节：从先天免疫和库普弗细胞的角度来看

基本信息

批准号：
18591500
负责人：
TAKEUCHI Dan
金额：
$ 2.57万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591500/
关键词：
Transplantation Regenerative Medicine Immunology Infection Shear Stress Cell Culture

项目摘要

After 30 mins of portal vein ischemia, rats underwent 70% or 90% of hepatectomy followed by reperfusion. These rats were divided to 2 groups and one group underwent splenic artery (SPA) ligation, resulting in four groups. Significant activation of STAT 3 was noted in the remnant liver of rats with 70% hepatectomy and SPA ligation group. Also, significant activation of NFκB was observed in the same group. Immunohistochemistry revealed decreased expression of MRP-2 in the remnant liver of rats with 90% hepatectomy group compared with 70% hepatectomy group.Cells in the sinusoidal space were isolated from the liver of hepatectomized rats. It was unable to obtain sufficient number of cells from the rats with 90% hepatectomy, due to the small amount of remnant liver. Adequate number of cells was obtained by putting cells from 3 rats together. Hepatic stellate cells (HSCs) from rats with 90% hepatectomy produced significantly higher amount of TGF-β compared with HSCs from rats with 70% hepate … More ctomy. Multiplication of hepatocytes from normal mice was suppressed when these cells were co-cultured with Kupffer cells from rats with 90% hepatectomy under stimulation with HGF, compared to hepatocytes cultured with Kupffer cells from rats with 70% hepatectomy. This effect was observed when cultured hepatocytes and Kupffer cells were allowed cell to cell contact.To investigate antimicrobial peptide production, mice underwent 70% hepatectomy with or without administration of dexamethasone ip. No significant difference was observed in pulmonary expression of murine beta defensin (MBD) -1, 3 mRNA between two groups when there was no bacterial infection. Pulmonary defensin mRNA expression was attenuated in mice with hepatectomy and dexamethasone administration when mice were infected with Pseudomonas aeruginosa intratracheally. Gene transfer of MBD-1 into 293 cells was successful and protein expression was confirmed by western blot analysis. These cells were more resistant Pseudomonas aeruginosa than wild type when TCID_<50> of each cell group was determined. Less

门静脉缺血 30 分钟后，大鼠接受 70% 或 90% 的肝切除，然后再灌注。这些大鼠被分为两组，其中一组接受脾动脉（SPA）结扎，形成四组。在 70% 肝切除和 SPA 结扎组大鼠的残余肝脏中发现 STAT 3 显着激活。此外，在同一组中还观察到 NFκB 的显着激活。免疫组化显示90%肝切除组大鼠残肝中MRP-2表达较70%肝切除组降低。从肝切除大鼠肝脏中分离肝窦间隙细胞。由于残余肝脏数量较少，无法从90%肝切除的大鼠中获得足够数量的细胞。将3只大鼠的细胞放在一起获得足够数量的细胞。与 70% 肝切除大鼠的肝星状细胞 (HSC) 相比，90% 肝切除大鼠的肝星状细胞 (HSC) 产生的 TGF-β 含量显着更高。与与 70% 肝切除大鼠的 Kupffer 细胞一起培养的肝细胞相比，当这些细胞与来自 90% 肝切除大鼠的 Kupffer 细胞在 HGF 刺激下共培养时，来自正常小鼠的肝细胞的增殖受到抑制。当培养的肝细胞和库普弗细胞进行细胞与细胞接触时，观察到了这种效应。为了研究抗菌肽的产生，小鼠接受了 70% 的肝切除术，腹腔注射或不注射地塞米松。无细菌感染时，两组小鼠肺部β防御素（MBD）-1、3 mRNA表达无显着差异。当小鼠气管内感染铜绿假单胞菌时，肝切除术和地塞米松给药小鼠的肺防御素 mRNA 表达减弱。 MBD-1 基因成功转移至 293 细胞中，蛋白质表达通过蛋白质印迹分析得到证实。当测定每个细胞组的TCID_<50>时，这些细胞对铜绿假单胞菌的抵抗力比野生型更强。较少的

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Excessive inflammation but decreased immunological response renders liver susceptible to infection in bile duct ligated mice

过度炎症但免疫反应降低导致胆管结扎小鼠肝脏易受感染

DOI：
发表时间：
2008
期刊：
J Surg Res 246
影响因子：
0
作者：
Morita Y;Yoshidome H;Kimura F;Takeuchi D;Miyazaki M;et.al.
通讯作者：
et.al.

Successful Treatment for Pancreatic Injury Presented with Pancreatic Ascites 2 Months after a Blunt Abdominal Trauma.

腹部钝性外伤 2 个月后出现胰腹水的胰腺损伤的成功治疗。

DOI：
发表时间：
期刊：
Hepatogastroenterology (In press)
影响因子：
0
作者：
Miyazaki M;Yoshidome H;Ohtsuka M;Kato A;Takeuchi D;et. al.;Morita Y.;Miyazaki M.;Takeuchi D.
通讯作者：
Takeuchi D.

Recent advance in the treatment of hilar cholangiocarcinoma: hepatectomy with vascular resection

肝门部胆管癌治疗新进展：肝切除联合血管切除