Lipid peroxidation and PAFacetylhydrolase activity of cerebrospinal fluid in patients with subarachnoid hemorrhage

蛛网膜下腔出血患者脑脊液脂质过氧化及PAF乙酰水解酶活性

• 批准号: 20591696
• 负责人: HIRASHIMA Yutaka
• 金额: $ 3万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591696/
• 关键词: 脳血管攣縮; くも膜下出血; 脂質過酸化; 脂質; 酵素; 脳神経疾患

We attempt to compare plasma PAF-AH activity and free forms of bio-marker of lipid peroxidation in cerebrospinal fluid (CSF) between patients with and without symptomatic vasospasm (SVS) after SAH. The identification of PAF-AH in CSF was performed by Western blotting. The genotype at position 279 of the plasma PAF-AH gene was determined. The activities of PAF-AH and the levels of free 8-iso-PGF_<2α> (free isoPs), free hydroxyoctadecadienoic acid (free HODE), and free hydroxyeicosatetraenoic acid (free HETE) in CSF were measured. The PAF-AH in CSF was confirmed to be only the plasma type. The genotype of the plasma PAF-AH was not different between patients with and without SVS. Free isoPs, free HODE, and free HETE showed higher values in patients without SVS in 0-4 days and 5-9 days after SAH. The PAF-AH activity also showed higher in patients without SVS in 0-4 days and 5-9 days after SAH. The associations between PAF-AH activity and free isoPs, and between PAF-AH activity and free HODE were significant.Oxidized lipids of lipoproteins and blood cell membranes produced by reactive oxygen species in CSF when SAH occurs may be the main source of lipid peroxidation. Plasma PAF-AH can hydrolize oxidized phospholipids, and may attenuate the spreading of lipid peroxidation and participate in defense mechanism against vasospasm after SAH

我们试图比较SAH后有无症状性血管痉挛(SVS)患者的血浆PAF-AH活性和脑脊液(CSF)中脂质过氧化生物标志物的游离形式。用免疫印迹法检测脑脊液中PAF-AH的表达。测定血浆PAF-AH基因第279位点的基因型。测定脑脊液中PAF-AH活性和游离8-iso-PGF_2、游离羟基十八碳二烯酸(α)、游离羟基二十碳四烯酸(Free HETE)的含量。脑脊液中PAF-AH仅为血浆型。血浆PAF-AH基因分型在SVS组与非SVS组间差异无统计学意义。在SAH后0~4天和5~9天，无SVS的患者游离同工酶、游离HODE和游离HETE的值较高。在SAH后0~4天和5~9天，无SVS的患者PAF-AH活性也较高。PAF-AH活性与游离同工酶、PAF-AH活性与游离HODE之间存在显著的相关性，提示SAH时脂蛋白的氧化脂质和脑脊液中活性氧产生的血细胞膜可能是SAH时脂质过氧化的主要来源。血浆PAF-AH可使氧化的磷脂水解化，可能抑制SAH后脂质过氧化的扩散，参与SAH后血管痉挛的防御机制

项目成果

新人薬剤師の不安の構造と自己教育力との関係

新药师焦虑结构与自我教育能力的关系

• 发表时间: 2009
• 期刊: YAKUGAKU ZASSHI 129
• 作者: 片野広之;ほか;平島豊
• 通讯作者: 平島豊

Hyperthermia during Ischemia Protects against Neuronal Death but Not Acute Brain Edema following Transient Forebrain Ischemia in Mice

缺血期间的高温可预防神经元死亡，但不能预防小鼠短暂前脑缺血后的急性脑水肿

• 发表时间: 2009
• 期刊: Biol. Pharm. Bull. 32
• 作者: 片野広之;南光徳偉;日向崇教;大蔵篤彦;岡雄一;間瀬光人;山田和雄;Doshi M
• 通讯作者: Doshi M

脳血管攣縮患者CSF中の酸化ストレスマーカー、8-イソプロスタンF2αとPAFアセチルヒドロラーゼ活性の測定

脑血管痉挛患者脑脊液氧化应激标志物8-异前列烷F2α及PAF乙酰水解酶活性测定

• 发表时间: 2008
• 作者: 片野広之;ほか;平島豊
• 通讯作者: 平島豊

ヒトII型PAFアセチルヒドロラーゼ遺伝子導入マウスによる神経細胞の長期的な抗脂質過酸化効果の検討.

使用人II型PAF乙酰水解酶转基因小鼠检查对神经元的长期抗脂质过氧化作用。

• 发表时间: 2010
• 作者: 道志勝;平島豊
• 通讯作者: 平島豊

脳血管攣縮患者CSF中の酸化ストレスマーカー、8-イソブロスクンF2aとPAF-アセチルヒドロラーゼ活性の測定

脑血管痉挛患者脑脊液氧化应激标志物8-isobroscune F2a及PAF-乙酰水解酶活性测定

• 发表时间: 2008
• 作者: 片野広之;ほか;平島豊;平島 豊
• 通讯作者: 平島 豊