Studies on the operative technique and the cytokines required to reproduce a normal bone-ligament junction in the knee ligament reconstruction

膝关节韧带重建中恢复正常骨韧带连接的手术技术和细胞因子的研究

• 批准号: 20591769
• 负责人: SOEJIMA Takashi
• 金额: $ 2.91万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591769/
• 关键词: 膝; 靭帯; 再建術; 骨.靭帯移行部; ACL; PCL; 靭帯再建術; 器官形成; 内軟骨骨化; 組織学的変化; コラーゲン; Sox-9; 骨靭帯移行部; 骨髄幹細胞; 骨-靭帯移行部; 膝前十字靭帯; 膝前十字靱帯

On immunohistological search of the rat, although ligament insertion site is completely bipolarized into the ligament cell which produces type I collagen at a viviparous period and the epiphysial cartilage cell which produces type II collagen, both cells re-arrange regularly to a fibrous direction after birth. In the boundary part, it seemed that a part of ligament cell transmuted into the cartilage cell and a part of cartilage cell transmuted into the ligament cell, with revelation of Sox9. (2) A direction of the anchor fiber came to agree with the mechanical axis of the grafted tendon when we changed the operation method from the conventional bone-tunnel method to our original method without bone-tunnel, although the tendon-bone junction was a fibrous union. (3) The cartilaginous bone-tendon junction through the layer of type II collagen which resembled to a normal bone-tendon junction was found by some individuals, when the bone marrow-derived mesenchymal stem cell which has multipotency and/or Sox 9 which is considered to be one of the key-factors by the experiment-1 were introduced into the our reconstruction model which established by the experiment-2.

在大鼠的免疫组织学研究中，虽然在胎生时期韧带插入部位完全双极化为产生I型胶原的韧带细胞和产生II型胶原的表皮软骨细胞，但在出生后，这两个细胞都有规律地向纤维方向重新排列。在边界部分，似乎一部分韧带细胞转化为软骨细胞，一部分软骨细胞转化为韧带细胞，显示Sox9。(2)虽然肌腱-骨连接处为纤维结合，但当我们将常规骨隧道法的操作方法改为不使用骨隧道的操作方法时，锚定纤维的方向与移植肌腱的机械轴一致。(3)将具有多能性的骨髓间充质干细胞和/或实验-1认为是关键因素之一的sox9引入实验-2建立的重建模型中，发现部分个体通过II型胶原层形成了类似于正常骨-肌腱连接的软骨骨-肌腱连接。

项目成果

ACL再建術における誘導枝を用いた機能的半腱様筋腱再生への試み

尝试在 ACL 重建手术中使用引导分支再生功能性半腱肌腱

• 发表时间: 2008
• 作者: 田渕幸祐;副島崇;村上秀孝;井上貴司;金澤知之進;野口幸志;加藤田倫宏;野山めぐみ;安永英樹;永田見生
• 通讯作者: 永田見生

Relationship between tnickness of the anteromedial bundle and thickness of the posterolateral bundle in the normal ACL

正常ACL前内侧束厚度与后外侧束厚度的关系

• 发表时间: 2011
• 期刊: Knee Surg Sports traumatol Athrosc
• 作者: Matsugaki T;Shiba N;Kohno S;Nikawa T;Hirasaka K;Okumura Y;Ishidoh K;Soejima T;Yoshimitsu K;Nagata K;Michihiro Katouda
• 通讯作者: Michihiro Katouda

ACL再建術における誘導枝を用いた半腱様筋腱再生:筋腹短縮と屈曲トルクについて

在 ACL 重建中使用引导分支进行半腱肌腱再生：关于肌腹缩短和屈曲扭矩

• 发表时间: 2010
• 作者: 村上秀孝;副島崇;井上貴司;金澤知之進;野口幸志;田渕幸祐;安永英樹;永田見生
• 通讯作者: 永田見生

Immunohistological changes of the tibial insertion site of anterior cruciate ligament in the organogenetic period of the rat

大鼠器官发生期前交叉韧带胫骨附着点的免疫组织学变化

• 发表时间: 2010
• 作者: Noyama M;Soejima T;Kanazawa T;Noguchi K;Tabuchi K;Nagata K;Megumi Noyama
• 通讯作者: Megumi Noyama

ACL再建術における誘導枝を用いた解剖学的半腱様筋腱再生の試み-3D-CTでの検討-

在 ACL 重建中使用引导分支进行解剖学半腱肌腱再生的尝试 - 使用 3D-CT 的研究 -

• 发表时间: 2009
• 作者: 村上秀孝;副島崇;井上貴司;金澤知之進;野口幸志;加藤田倫宏;田渕幸祐;野山めぐみ;安永英樹;永田見生
• 通讯作者: 永田見生