Anesthesia model provoking emergence by laser beam irradiation

该项目旨在制造带有与绿色荧光蛋白（GFP）融合的α2A肾上腺素受体（α2A AR）的转基因小鼠，该受体源自水母维多利亚水母。我们成功地制备了α2A AR与GFP融合的转基因小鼠。该融合蛋白具有与野生型相同的功能和表达。这种转基因小鼠将成为分析 α2A 肾上腺素受体亚型的发育、分布和功能的重要分子工具。

This project is to make transgenic mouse with alpha2A adrenergic receptor (alpha2A AR) fused with green fluorescent protein (GFP), derived from jellyfish, Aequorea Victoria. We are successfully able to make transgenic mouse with alpha2A AR fused with GFP. Thisfusion protein has the same function and expression as the wild type has. This transgenicmouse will be a great molecular tool to analyze the development, distribution, and function of alpha2A adrenoceptor subtype.