COPAS FP-PRO 500 Flow Cytometer

COPAS FP-PRO 500 流式细胞仪

• 批准号: 9075758
• 负责人: Jennifer L Garrison
• 金额: $ 49.1万
• 依托单位: BUCK INSTITUTE FOR RESEARCH ON AGING
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-01 至 2017-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075758
• 关键词: Aging; Aging-Related Process; Biochemical; Biological Markers; California; Cells; Complex; Data; Disease; Disease model; Experimental Designs; Flow Cytometry; Funding; Grant; Health; Housing; Human; Image; Institutes; Life; Mammalian Cell; Molecular Genetics; Morphology; Neurodegenerative Disorders; Obesity; Organism; Principal Investigator; Process; Proteins; Research; Research Institute; Research Personnel; Research Project Grants; San Francisco; Sorting - Cell Movement; Staging; Stem cells; Techniques; United States National Institutes of Health; Universities; age related; gene product; improved; instrument; instrumentation; mitochondrial dysfunction; novel; particle; regenerative

 DESCRIPTION (provided by applicant): The overall objective of research at the Buck Institute for Research on Aging is to understand mechanisms of aging and its associated diseases in order to extend the healthy years of human life. Research at the Buck Institute is reaching the stage at which techniques perfected for isolated cells can now be applied to more complex multicellular organisms. This shared instrumentation proposal seeks funds for the purchase of a Complex Object Parametric Analyzer and Sorter (COPAS(tm)) FP PRO flow cytometer. This instrument will be housed and maintained at the Buck Institute's Morphology & Imaging Core and will be used by 10 independent principal investigators from the Buck Institute and 3 external investigators from the University of California, San Francisco. Together, these researchers bring together 16 NIH-funded grants, all of which will benefit from the ability to analyze and sort large particles. The unique capability of the COPAS to sort objects that are too large or too fragile for conventional flow cytometry allows analysis and sorting of whole multicellular organisms, intact cell clusters, and large mammalian cells. The data obtained from the requested instrument will be critical to understand various disease models and paradigms. Indeed, these research projects encompass the study of neurodegenerative diseases processes, the regenerative capacity of stem cells, the identification of novel protein targets or biomarkers for obesity, and the identification of compounds that modulate mitochondrial dysfunction and proteostasis in aging and age-related diseases. These projects are unified in using molecular, genetic, and biochemical techniques to ask how specific gene products influence cellular and organismal aging processes. The majority of these projects are not possible without the use of this instrument. Overall, the acquisition of the COPAS FP PRO flow cytometer will greatly improve the throughput and experimental design of projects that target important aspects of human health and healthspan.

 描述（由适用提供）：Buck衰老研究所研究的总体目标是了解衰老及其相关疾病的机制，以延长人类生活的健康年份。 Buck Institute的研究正达到该阶段的阶段，现在可以将适合孤立细胞的技术应用于更复杂的多细胞生物。该共享的仪器提案寻求资金购买复杂的对象参数分析仪和分类器（COPAS（TM））FP Pro流式细胞仪。该工具将在Buck Institute的形态和成像核心中安置和维护，并将由Buck Institute的10位独立首席研究人员和加利福尼亚大学旧金山分校的3名外部调查员使用。这些研究人员一起汇集了16个NIH资助的赠款，所有这些都将从分析和分类大型的能力中受益 颗粒。 COPA的独特能力排序太大或太脆弱的对象 常规的流式细胞仪允许对整个多细胞组织，完整的细胞簇和大型哺乳动物细胞进行分析和分类。从请求的仪器中获得的数据对于了解各种疾病模型和范式至关重要。实际上，这些研究项目包括对神经退行性疾病过程的研究，干细胞的再生能力，对物体的新蛋白质靶标或生物标志物的鉴定，以及调节线粒体功能障碍和蛋白质之间的化合物的鉴定。这些项目是在使用分子，遗传和生化技术来询问特定基因产物如何影响细胞和有机衰老过程的情况下统一的。如果没有使用该工具，这些项目大多数是不可能的。总体而言，COPAS FP PRO流式细胞仪的获取将大大改善针对人类健康和健康范围重要方面的项目的吞吐量和实验设计。