COPAS FP-PRO 500 Flow Cytometer

COPAS FP-PRO 500 流式细胞仪

• 批准号: 9075758
• 负责人: Jennifer L Garrison
• 金额: $ 49.1万
• 依托单位: BUCK INSTITUTE FOR RESEARCH ON AGING
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-01 至 2017-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075758
• 关键词: Aging; Aging-Related Process; Biochemical; Biological Markers; California; Cells; Complex; Data; Disease; Disease model; Experimental Designs; Flow Cytometry; Funding; Grant; Health; Housing; Human; Image; Institutes; Life; Mammalian Cell; Molecular Genetics; Morphology; Neurodegenerative Disorders; Obesity; Organism; Principal Investigator; Process; Proteins; Research; Research Institute; Research Personnel; Research Project Grants; San Francisco; Sorting - Cell Movement; Staging; Stem cells; Techniques; United States National Institutes of Health; Universities; age related; gene product; improved; instrument; instrumentation; mitochondrial dysfunction; novel; particle; regenerative

 DESCRIPTION (provided by applicant): The overall objective of research at the Buck Institute for Research on Aging is to understand mechanisms of aging and its associated diseases in order to extend the healthy years of human life. Research at the Buck Institute is reaching the stage at which techniques perfected for isolated cells can now be applied to more complex multicellular organisms. This shared instrumentation proposal seeks funds for the purchase of a Complex Object Parametric Analyzer and Sorter (COPAS(tm)) FP PRO flow cytometer. This instrument will be housed and maintained at the Buck Institute's Morphology & Imaging Core and will be used by 10 independent principal investigators from the Buck Institute and 3 external investigators from the University of California, San Francisco. Together, these researchers bring together 16 NIH-funded grants, all of which will benefit from the ability to analyze and sort large particles. The unique capability of the COPAS to sort objects that are too large or too fragile for conventional flow cytometry allows analysis and sorting of whole multicellular organisms, intact cell clusters, and large mammalian cells. The data obtained from the requested instrument will be critical to understand various disease models and paradigms. Indeed, these research projects encompass the study of neurodegenerative diseases processes, the regenerative capacity of stem cells, the identification of novel protein targets or biomarkers for obesity, and the identification of compounds that modulate mitochondrial dysfunction and proteostasis in aging and age-related diseases. These projects are unified in using molecular, genetic, and biochemical techniques to ask how specific gene products influence cellular and organismal aging processes. The majority of these projects are not possible without the use of this instrument. Overall, the acquisition of the COPAS FP PRO flow cytometer will greatly improve the throughput and experimental design of projects that target important aspects of human health and healthspan.

 描述(由申请人提供)：巴克老龄化研究所研究的总体目标是了解衰老及其相关疾病的机制，以延长人类生命的健康年限。巴克研究所的研究正在达到这样一个阶段，即完善的分离细胞技术现在可以应用于更复杂的多细胞生物体。这项共享仪器提案寻求资金，用于购买复杂对象参数分析仪和分类器(COPAS(Tm))FP PRO流式细胞仪。该仪器将安装在巴克研究所的形态和成像核心，并由来自巴克研究所的10名独立首席调查人员和来自加州大学旧金山分校的3名外部调查人员使用。这些研究人员总共带来了16笔由美国国立卫生研究院资助的赠款，所有这些资金都将受益于分析和分类大量 粒子。COPA的独特能力，可以对太大或太脆弱的对象进行分类 传统的流式细胞术允许对整个多细胞生物体、完整的细胞团和大型哺乳动物细胞进行分析和分选。从所请求的仪器获得的数据对于理解各种疾病模型和范例至关重要。事实上，这些研究项目包括研究神经退行性疾病的过程、干细胞的再生能力、识别肥胖的新蛋白质靶点或生物标记物，以及识别在衰老和老年相关疾病中调节线粒体功能障碍和蛋白稳定的化合物。这些项目在使用分子、遗传和生化技术来研究特定基因产品如何影响细胞和生物衰老过程方面是统一的。如果不使用这一工具，这些项目中的大多数都是不可能的。总体而言，收购Copas FP PRO流式细胞仪将极大地提高针对人类健康和健康跨度的重要方面的项目的吞吐量和实验设计。