COPAS FP-PRO 500 Flow Cytometer

COPAS FP-PRO 500 流式细胞仪

• 批准号: 9075758
• 负责人: Jennifer L Garrison
• 金额: $ 49.1万
• 依托单位: BUCK INSTITUTE FOR RESEARCH ON AGING
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-01 至 2017-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075758
• 关键词: Aging; Aging-Related Process; Biochemical; Biological Markers; California; Cells; Complex; Data; Disease; Disease model; Experimental Designs; Flow Cytometry; Funding; Grant; Health; Housing; Human; Image; Institutes; Life; Mammalian Cell; Molecular Genetics; Morphology; Neurodegenerative Disorders; Obesity; Organism; Principal Investigator; Process; Proteins; Research; Research Institute; Research Personnel; Research Project Grants; San Francisco; Sorting - Cell Movement; Staging; Stem cells; Techniques; United States National Institutes of Health; Universities; age related; gene product; improved; instrument; instrumentation; mitochondrial dysfunction; novel; particle; regenerative

 DESCRIPTION (provided by applicant): The overall objective of research at the Buck Institute for Research on Aging is to understand mechanisms of aging and its associated diseases in order to extend the healthy years of human life. Research at the Buck Institute is reaching the stage at which techniques perfected for isolated cells can now be applied to more complex multicellular organisms. This shared instrumentation proposal seeks funds for the purchase of a Complex Object Parametric Analyzer and Sorter (COPAS(tm)) FP PRO flow cytometer. This instrument will be housed and maintained at the Buck Institute's Morphology & Imaging Core and will be used by 10 independent principal investigators from the Buck Institute and 3 external investigators from the University of California, San Francisco. Together, these researchers bring together 16 NIH-funded grants, all of which will benefit from the ability to analyze and sort large particles. The unique capability of the COPAS to sort objects that are too large or too fragile for conventional flow cytometry allows analysis and sorting of whole multicellular organisms, intact cell clusters, and large mammalian cells. The data obtained from the requested instrument will be critical to understand various disease models and paradigms. Indeed, these research projects encompass the study of neurodegenerative diseases processes, the regenerative capacity of stem cells, the identification of novel protein targets or biomarkers for obesity, and the identification of compounds that modulate mitochondrial dysfunction and proteostasis in aging and age-related diseases. These projects are unified in using molecular, genetic, and biochemical techniques to ask how specific gene products influence cellular and organismal aging processes. The majority of these projects are not possible without the use of this instrument. Overall, the acquisition of the COPAS FP PRO flow cytometer will greatly improve the throughput and experimental design of projects that target important aspects of human health and healthspan.

 描述（由申请人提供）：巴克衰老研究所研究的总体目标是了解衰老及其相关疾病的机制，以延长人类的健康寿命。巴克研究所的研究已达到这样的阶段：针对分离细胞的完善技术现在可以应用于更复杂的多细胞生物体。该共享仪器提案寻求资金购买复杂对象参数分析仪和分类器 (COPAS(tm)) FP PRO 流式细胞仪。该仪器将由巴克研究所的形态学和成像核心安置和维护，由巴克研究所的 10 名独立首席研究员和来自加州大学旧金山分校的 3 名外部研究人员使用。这些研究人员汇集了 16 项 NIH 资助的资助项目，所有这些资助项目都将受益于分析和分类大型研究的能力。 颗粒。 COPAS 具有对太大或太脆弱的物体进行分类的独特功能 传统的流式细胞术可以对整个多细胞生物体、完整的细胞簇和大型哺乳动物细胞进行分析和分选。从所需仪器获得的数据对于理解各种疾病模型和范例至关重要。事实上，这些研究项目包括神经退行性疾病过程的研究、干细胞的再生能力、肥胖的新蛋白质靶标或生物标志物的鉴定，以及调节衰老和年龄相关疾病中线粒体功能障碍和蛋白质稳态的化合物的鉴定。这些项目统一使用分子、遗传和生化技术来探究特定基因产物如何影响细胞和有机体的衰老过程。如果不使用该工具，大多数项目都是不可能实现的。总体而言，购买 COPAS FP PRO 流式细胞仪将极大地提高针对人类健康和健康寿命重要方面的项目的通量和实验设计。