Development of new screening system for anti-angiogenic drugs using tumor associated endothelial cells derived from renal cell carcinoma

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To develope new screening system for anti-angiogenic drugs, we isolated normal endothelial cell (NEC) and tumor endothelial cell (TEC) from human renal cell carcinoma xenografts and from dermis of normal mice, respectively. We analyzed the difference between TEC and NEC and investigate the potential of TEC for the screening system which reflect tumor microenvironment.Especially, we focused on several genes which were upregulated in TEC compared to NEC by DNA microarray analysis.We found these genes had strong association with abnormal phenotypes of TECs such as drug resistance, high viability, and migration.The specific phenotype in mouse TEC were also found in human TEC isolated from human renal cell carcinoma.These results enable us to approach new anti-angiogenic drug screening system and new anti-angiogenic therapy.