Discovery of diagonositc and therapeutic biomarker by circulating tumor endothelial cells in metastatic renal cell carcinoma

我们鉴定了几个标记(PTGIR、Biglycan、LOX、CXCR7等)。实时定量聚合酶链式反应检测转移性肾癌循环内皮细胞在小鼠肿瘤内皮细胞中的高表达。我们从人肾癌组织中分离出人TEC，并从6例患者的正常肾组织中分离出人正常内皮细胞。在所有配对病例中，PTGIR、Biglycan、LOX和CXCR7在人TEC中的表达水平均显著高于人NEC。提示CEC可能是肾癌的一个有用的生物标志物。

We identified several markers (PTGIR, Biglycan, LOX, CXCR7 etc. ) highly expressed in mouse tumor endothelial cell (TEC) by real-time PCR of circulating endothelial cell (CEC) in metastatic renal cell carcinoma(RCC). We isolated human TEC from human RCC tissues and human normal endothelial cell (NEC) from normal kidney tissues of six patients. PTGIR, Biglycan, LOX and CXCR7 expression levels were significantly higher in human TEC than in human NEC for all paired cases. These results suggested that CEC might be a useful biomarker of RCC.