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Analysis of expression pattern of the gene which is related to the radiation sensitivity in urothelial cancer and its clinical application

尿路上皮癌放射敏感性相关基因表达模式分析及临床应用

基本信息

批准号：
15390482
负责人：
SHINOHARA Nobuo
金额：
$ 5.12万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

From the present project, the following results were obtained.1.Initially, we evaluated radiation sensitivity in the nude mice transplanted with human bladder cancer cell lines. Utilizing this model, we set up the radiation exposure condition (single time irradiation of 5 Gy) and confirmed that tumor growth delayd reaction could be evaluated as an endpoint of radiation sensitivity.2.The establishment of Xenogarft model : Tumor growth was observed in 18 mice (60%, 18/30). Histologically, 15 of the18 were epithelial carcinomas similar to the original tumors, resulting in a 50% (15/30) take rate. No correlation was found between the tumor take rate and the clinicopathologic features, TP53 mutational status, or Ki67 LI of the patients' tumors. Of the 15 xenografts, 11 xenografts were passed from 3 to 10 generations. TP53 mutational status remained stable during the passages, though the Ki67 LI slightly increased in the majority of xenografts. Specific growth delay after irradiation in the 4 xenografts was observed independent of the original tumor growth speed and Ki67 LI.3.Radiological growth delay assay : To assess the usefulness of these xenografts for therapeutic simulation, we performed radiological growth-delay assay in 4 xenografts and examined the relation between the responses and the biological parameters of xenograft tumors. The radiation response considerably varied among the 4 models. Absolute growth delay ranged from 20.3+2.4 (mean+standard error) to 76.4±7.4 days. Specific growth delay (SGD) was not associated with in vivo tumor growth speed or the Ki67 Il. The smallest SGD value was observed in the No.3 model which harbored TP53 gene mutation.

从目前的项目中，得到了以下结果：最初，我们评估了裸鼠移植人类膀胱癌细胞系的辐射敏感性。利用该模型，我们设置了5 Gy单次照射的照射条件，并证实肿瘤生长延迟反应可以作为辐射敏感性的评价终点。Xenogarft模型的建立：18只小鼠（60%,18/30）观察到肿瘤生长。组织学上，18例中有15例为上皮性癌，与原肿瘤相似，发生率为50%（15/30）。肿瘤发生率与患者肿瘤的临床病理特征、TP53突变状态、Ki67 LI均无相关性。15例异种移植物中，11例为3 ~ 10代传代。TP53突变状态在传代过程中保持稳定，尽管Ki67 LI在大多数异种移植物中略有增加。4例异种移植物辐照后特异性生长延迟与原肿瘤生长速度和ki67li无关。放射生长延迟试验：为了评估这些异种移植物在治疗模拟中的有效性，我们对4个异种移植物进行了放射生长延迟试验，并检查了反应与异种移植物肿瘤生物学参数之间的关系。4种模式的辐射响应差异很大。绝对生长延迟从20.3+2.4（平均值+标准误差）到76.4±7.4天。特异性生长延迟（Specific growth delay， SGD）与体内肿瘤生长速度或ki67il无关。在TP53基因突变的3号模型中，SGD值最小。

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Gene-expression profile changes correlated with tumor progression and lymph node metastasis in Esophageal cancer

DOI：
10.1158/1078-0432.ccr-04-0048
发表时间：
2004-06-01
期刊：
CLINICAL CANCER RESEARCH
影响因子：
11.5
作者：
Tamoto, E;Tada, M;Katoh, H
通讯作者：
Katoh, H

進行性尿路上皮癌に対するTIN(Paclitaxel, Ifosfamide, Nedaplatin)療法

TIN（紫杉醇、异环磷酰胺、奈达铂）治疗晚期尿路上皮癌

DOI：
发表时间：
2004
期刊：
癌と化学療法 31(8)
影响因子：
0
作者：
鈴木信;篠原信雄;他
通讯作者：
他

Prediction of lymph node metastasis by analysis of gene expression profiles in non-small cell lung cancer.

DOI：
10.1016/j.jss.2004.06.002
发表时间：
2004-11
期刊：
The Journal of surgical research
影响因子：
0
作者：
M. Takada;M. Tada;Eiji Tamoto;Akiko Kawakami;Katsuhiko Murakawa;G. Shindoh;Ken-ichi Teramoto;A. Matsunaga;K. Komuro;M. Kanai;Y. Fujiwara;K. Shirata;Norihiro Nishimura;M. Miyamoto;S. Okushiba;S. Kondo;J. Hamada;H. Katoh;T. Yoshiki;T. Moriuchi
通讯作者：
M. Takada;M. Tada;Eiji Tamoto;Akiko Kawakami;Katsuhiko Murakawa;G. Shindoh;Ken-ichi Teramoto;A. Matsunaga;K. Komuro;M. Kanai;Y. Fujiwara;K. Shirata;Norihiro Nishimura;M. Miyamoto;S. Okushiba;S. Kondo;J. Hamada;H. Katoh;T. Yoshiki;T. Moriuchi

集学的治療における膀胱全摘除術と尿路変向術

多学科治疗中的根治性膀胱切除术和尿流改道术