The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization

使用荧光原位杂交证明胆道闭锁中母体微嵌合现象

• 批准号: 20592089
• 负责人: HAYASHIDA Makoto
• 金额: $ 3万
• 依托单位: Jichi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592089/
• 关键词: 胆道閉鎖症; 成因; マイクロキメリズム

Biliary atresia (BA) is a cholestatic disease of unknown etiology. It has recently been suggested that GVHD caused by microchimerism is an etiology in the development of autoimmune disease. Moreover, the liver is a frequent target organ of GVHD. The aim of this study is to identify the presence and extent of maternal microchimerism and to determine whether it play a role in the etiology of BA. The liver biopsy specimens of 6 male BA patients (BA group) and 6 males with other liver diseases (non-BA group) were assayed for X- and Y-chromosome using fluorescent in situ hybridization (FISH). The cells with two sex chromosomes in the nuclei were counted. Cells with one X- and one Y-chromosomes were considered to be host cells and those with two X-chromosome were considered to be of maternal origin. The frequency of cells with XX chromosomes per 1,000 host cells in the BA group and the non-BA group were 3.00±0.75 and 0.99±0.50, respectively. (P=0.005). The presence of female cells in the liver of male BA patients was significantly higher than in males with other liver disease. Maternal microchimerism is therefore suggested to contribute to the pathogenesis of BA.

胆道闭锁（BA）是一种未知病因的胆固性疾病。最近有人提出，由微chimerismismis造成的GVHD是自身免疫性疾病发展的一种病因。此外，肝脏通常是GVHD的靶向器官。这项研究的目的是确定孕产妇微思维主义的存在和程度，并确定它是否在BA病因中起作用。使用荧光原位杂交（FISH）分配了6例男性BA患者（BA）组的肝活检标本（BA）组（BA）组（BA）组（BA）组（BA）组（非BA组）。计数核中有两个性别染色体的细胞。一个X-和一个Y染色体的细胞被认为是宿主细胞，并且具有两个X染色体的细胞被认为是母性的。在BA组和非BA组中，每1,000个宿主细胞的XX染色体的细胞频率分别为3.00±0.75和0.99±0.50。 （p = 0.005）。雄性BA患者肝脏中雌性细胞的存在明显高于其他肝病的男性。因此，建议孕产妇的微思维主义有助于BA的发病机理。

项目成果

胆道閉鎖症術後における肺血流異常の検討

胆道闭锁术后肺血流异常的检查

• 发表时间: 2010
• 作者: Hiyama E;et al.;林田真;檜山英三;林田真;檜山英三;林田真
• 通讯作者: 林田真

重症心身障害児に対する小児外科の関わり～当院にて手術を行った重症心身障害児の検討～

小儿外科对严重精神和身体残疾儿童的参与 - 在我院接受手术的严重精神和身体残疾儿童的检查 -

• 发表时间: 2010
• 作者: 檜山英三;他;林田真
• 通讯作者: 林田真

The prevalence and clinical significance of autoantibodies following living donor liver transplantation in children

儿童活体肝移植后自身抗体的流行情况及临床意义

• 发表时间: 2009
• 作者: 本多昌平;他;林田真;山岡裕明;林田真
• 通讯作者: 林田真

Association of lymphocyte crossmatch and outcome of intestinal transcription in swine

猪淋巴细胞交叉配型与肠道转录结果的关联

• 发表时间: 2010
• 作者: 谷本圭司;他;林田真;林田真
• 通讯作者: 林田真

Surgical complication after living donor liver transplantation in patients with biliary atresia : a relatively high incidence of portal vein complications.

胆道闭锁患者活体肝移植后的手术并发症：门静脉并发症的发生率相对较高。

• 发表时间: 2009
• 期刊: Pediatr Surg Int 25(9)
• 作者: Takahashi Y;Nishimoto Y;Matsuura T,Hayashida M;Tajiri T;Soejima Y;Taketomi A;Maehara Y;Taguchi T
• 通讯作者: Taguchi T