Investigation on cardiac function during anaphylactic shock

过敏性休克时心功能的调查

• 批准号: 20592131
• 负责人: SHIBAMOTO Toshishige
• 金额: $ 3.16万
• 依托单位: Kanazawa Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592131/
• 关键词: アナフィラキシーショック; 心機能; 血液交差灌流心臓; Emax; 冠血管収縮; 心アナフィラキシー; 血液交叉灌流心臓; 心収縮性; 冠動脈血流; βアドレナリン受容体阻害剤; dP/dt max; 循環ショック; ラット; 血液交差灌流; 摘出灌流心臓; dP; dtmax; eEmax

The aim of the present study was to determine the effects of anaphylaxis on the left ventricular function in both anesthetized rats and isolated perfused rat hearts. The rats were sensitized with ovalbumin. In anesthetized rats, Emax, an index of cardiac contractility did not decrease but increased at 15 min after antigen injection, suggesting that cardiac contractility did not decrease but increased during sustained hypotension due to systemic anaphylaxis in anesthetized rats. In the study using isolated hearts that were excised from Wister rats sensitized with ovalbumin, and perfused at constant flow with blood in a cross-circulated fashion. Cardiac contractility was evaluated by positive maximum rate of changes in left ventricular pressure (dP/dt_<max>) and end-systolic pressure volume relationships (ESPVRs). After antigen, the coronary vascular resistance increased four times the baseline only transiently after antigen. The dP/dt_<max> decreased transiently along with the decrease in coronary flow. The ESPVRs measured at 10 min after antigen did not change from the baseline. The results from perfused heart experiments suggest that cardiac contractility decreased only transiently along with a decrease in coronary blood flow during cardiac anaphylaxis of isolated blood-perfused rat hearts. In conclusion, cardiac contractility may not decrease, but rather increase later than 10 min after antigen exposure. However, the cardiac anaphylactic reaction itself is accompanied by a transient flow-dependent decrease in cardiac contractility.

本研究的目的是确定过敏反应对麻醉大鼠和离体灌流大鼠心脏左心室功能的影响。用卵白蛋白致敏大鼠。在麻醉大鼠中，Emax，心脏收缩力的指数没有减少，但在抗原注射后15分钟增加，这表明心脏收缩力没有减少，但在麻醉大鼠由于全身过敏反应持续低血压增加。在这项研究中，使用了从用卵白蛋白致敏的Wister大鼠中切除的离体心脏，并以恒定流量以交叉循环的方式用血液灌注。通过左心室压力的正最大变化率（dP/dt_<max>）和收缩末期压力容积关系（ESPVR）来评价心脏收缩力。在抗原后，冠状动脉血管阻力增加4倍的基线只是短暂的抗原后。dP/dt_<max>随冠脉流量的减少而沿着一过性降低。在抗原后10分钟测量的ESPVR与基线相比没有变化。灌注心脏实验的结果表明，心脏收缩力下降只是短暂的沿着在离体血液灌注大鼠心脏的心脏过敏反应期间，在冠状动脉血流量的减少。总之，心肌收缩力可能不会降低，而是在抗原暴露后10分钟后增加。然而，心脏过敏反应本身伴随着短暂的血流依赖性心肌收缩力下降。

项目成果

Pulmonary vasoconstrictive and bronchoconstrictive responses to anaphylaxis are weakened via β_2-adrenoceptor activation by endogenous epinephrine in anesthetized rats.

麻醉大鼠中内源性肾上腺素激活β_2-肾上腺素受体，从而减弱了对过敏反应的肺血管收缩和支气管收缩反应。

• 发表时间: 2011
• 期刊: Anesthesiology
• 影响因子: 8.8
• 作者: Shibamoto T;Tsutsumi M;Kuda Y;Ohmukai C;Zhang W;Kurata Y;Wei Zhang
• 通讯作者: Wei Zhang

Portacaval shunting attenuates portal hypertension and systemic hypotension in rat anaphylactic shock

• DOI: 10.1007/s12576-010-0123-x
• 发表时间: 2011-03
• 期刊: The Journal of Physiological Sciences
• 作者: C. Kamikado;T. Shibamoto;Wei Zhang;Y. Kuda;Chieko Ohmukai;Y. Kurata

Pulmonary vasoconstrictive and bronchoconstrictive responses to anaphylaxis are weakened via β 2-adrenoceptor activation by endogenous epinephrine in anesthetized rats.

麻醉大鼠中内源性肾上腺素激活β2-肾上腺素受体，从而减弱了对过敏反应的肺血管收缩和支气管收缩反应。

• 发表时间: 2011
• 期刊: Anesthesiology. 114(3)
• 作者: Zhang W;Shibamoto T;Kuda Y;Ohmukai C;Kurata Y
• 通讯作者: Kurata Y

Mast cells are not involved in the ischemia-reperfusion injury in perfused rat liver.

• DOI: 10.1016/j.jss.2010.11.900
• 发表时间: 2012-05
• 期刊: The Journal of surgical research
• 作者: T. Shibamoto;M. Tsutsumi;Y. Kuda;Chieko Ohmukai;Wei Zhang;Y. Kurata

血液交叉灌流系を用いた心アナフィラキシーの病態解明:心収縮性と冠血流への影響

使用血液交叉灌注系统阐明心脏过敏反应的病理学：对心肌收缩力和冠状动脉血流的影响

• 发表时间: 2011
• 作者: Shibamoto T;Tsutsumi M;Kuda Y;Ohmukai C;Zhang W;Kurata Y;Wei Zhang;Chiaki Kamikado;Toshishige Shibamoto;倉田康孝(芝本利重)
• 通讯作者: 倉田康孝(芝本利重)