Role of bacterial aggregation in the periapical lesions

细菌聚集在根尖周病变中的作用

• 批准号: 20592249
• 负责人: YONEDA Masahiro
• 金额: $ 2.83万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592249/
• 关键词: 根尖性歯周炎; TGF; マクロファージ; プロバイオティクス; 細菌培養検査; TGF-β1; 口臭

In the periapical lesions, especially the refractory one, some specific bacteria are known to produce biofilms. They are also known to make the treatment more difficult. Recently, it is reported that Fusobacterium nucleatum is associated with flare-ups (acute phase after treatment), and the importance of the bacterium is often discussed. On the other hand, host conditions are also considered to be the important factor of flare-ups.We examined the role of TGF-・1 in the development of periapical lesions. When ofloxacin was applied to the rat periapical lesions, macrophages manifesting TGF-・1 increased and decreased thereafter. Bone formation was observed at the periapical area. Decrease of expression of IL-1・ and IL-1・・ were observed in ofloxacin-applied group. On the other hand, macrophages continued to express these cytokines in control group. From these results, the duration and amount of TGF-・1 expression is considered to affect the healing process. It is also considered that temporary increase of macrophages expressing TGF-・1 is playing an important role in the bone formation at the healing of periapical lesions.We also reported a case with halitosis associated with inappropriate endodontic treatment. We are now performing an experiment with clinical isolates from periapical periodontitis lesions, and the ability of coaggregation of the clinical isolates are being compared with the laboratory strains. We will reveal the host responses in periapical lesions. We did a work with probiotics, which changed the oral flora and improved the general health.

在根尖周病变中，特别是难治性根尖周病变中，已知某些特定的细菌可产生生物膜。他们也知道，使治疗更加困难。最近，据报道，具核梭杆菌与突发（治疗后的急性期）有关，细菌的重要性经常被讨论。另一方面，宿主环境也被认为是根尖周病变发生的重要因素，我们研究了TGF-·1在根尖周病变发生发展中的作用。当氧氟沙星应用于大鼠根尖周病变时，表达TGF-1的巨噬细胞增加，随后减少。在根尖周区域观察到骨形成。氧氟沙星组IL-1·、IL-1··表达减少。而对照组巨噬细胞则持续表达这些细胞因子。根据这些结果，TGF-1表达的持续时间和量被认为影响愈合过程。我们还报告了1例因根管治疗不当引起的口臭病例。我们现在正在进行一项实验，从根尖周炎病变的临床分离株，并与实验室菌株的共聚集能力的临床分离株进行比较。我们将揭示根尖周病变的宿主反应。我们用益生菌做了一项工作，它改变了口腔植物群，改善了整体健康状况。

项目成果

長期間悩まされた口腔異常感が口腔乾燥に対する処置により消失した症例

困扰他已久的口腔异常，经过口干治疗后消失了。

• 发表时间: 2010
• 期刊: 日本口腔ケア誌 4(1)
• 作者: 米田雅裕;峰真理子;鈴木奈央;内田初美;黒木まどか;廣藤卓雄
• 通讯作者: 廣藤卓雄

Lactobacillus salivalius WB21株を利用したプロバイオティクスの口臭改善効果

唾液乳杆菌WB21菌株益生菌改善口臭的效果

• 发表时间: 2010
• 期刊: 日口臭誌 1(1)
• 作者: 岩元知之;鈴木奈央;米田雅裕;田邊一成;平田晴久;中谷清吾;竹下徹;内藤徹;山下喜久;山田和彦;岡田一三;畑野優子;桝尾陽介;藤本暁江;廣藤卓雄
• 通讯作者: 廣藤卓雄

福岡歯科大学における臨床研修歯科医の針刺し・切創防止についての意識調査

福冈齿科大学临床实习牙医预防针刺伤和割伤意识调查

• 发表时间: 2010
• 期刊: 日歯教誌 26(2)
• 作者: 米田雅裕;永井淳;清水博史;内田竜司;尾崎正雄;本川 渉;松浦正朗;廣藤卓雄
• 通讯作者: 廣藤卓雄

福岡歯科大学医科歯科総合病院における針刺し・切創等の分析および対策の検討

福冈齿科大学医科齿科综合医院的针刺、割伤等分析及对策研究

• 发表时间: 2009
• 期刊: 日歯保存誌 52(2)
• 作者: Masahiro Yoneda;Hatsumi Uchida;Nao Suzuki;Mariko Mine;Tomoyuki Iwamoto;Yosuke Masuo;Toru Naito;Yuko Hatano;Takao Hirofuji;山下絵美;米田雅裕,泉利雄,鈴木奈央,内藤徹,山田和彦,岡田一三,岩元知之,桝尾陽介,小島寛,阿南壽,廣藤卓雄
• 通讯作者: 米田雅裕,泉利雄,鈴木奈央,内藤徹,山田和彦,岡田一三,岩元知之,桝尾陽介,小島寛,阿南壽,廣藤卓雄

口臭治療のA to Z(3)真性口臭症の治療における歯科衛生士の役割

口臭治疗A to Z (3) 牙科保健员在真性口臭治疗中的作用

• 发表时间: 2008
• 期刊: デンタルハイジーン 28(9)
• 作者: 鈴木奈央;米田雅裕;内藤徹;吉兼透;岩元知之;廣藤卓雄;峰真理子,米田雅裕,鈴木奈央,内藤徹,岡田一三,内田初美,吉兼透,島野裕一,岩元知之,廣藤卓雄;米田雅裕,鈴木奈央,内藤徹,山田和彦,岡田一三,島野裕一,岩元知之,廣藤卓雄;米田雅裕,鈴木奈央,峰真理子,内藤徹,廣藤卓雄;鈴木奈央,米田雅裕,峰真理子,内藤徹,廣藤卓雄;峰真理子,米田雅裕,鈴木奈央,内藤徹,廣藤卓雄
• 通讯作者: 峰真理子,米田雅裕,鈴木奈央,内藤徹,廣藤卓雄