Role of bacterial aggregation in the periapical lesions

细菌聚集在根尖周病变中的作用

• 批准号: 20592249
• 负责人: YONEDA Masahiro
• 金额: $ 2.83万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592249/
• 关键词: 根尖性歯周炎; TGF; マクロファージ; プロバイオティクス; 細菌培養検査; TGF-β1; 口臭

In the periapical lesions, especially the refractory one, some specific bacteria are known to produce biofilms. They are also known to make the treatment more difficult. Recently, it is reported that Fusobacterium nucleatum is associated with flare-ups (acute phase after treatment), and the importance of the bacterium is often discussed. On the other hand, host conditions are also considered to be the important factor of flare-ups.We examined the role of TGF-・1 in the development of periapical lesions. When ofloxacin was applied to the rat periapical lesions, macrophages manifesting TGF-・1 increased and decreased thereafter. Bone formation was observed at the periapical area. Decrease of expression of IL-1・ and IL-1・・ were observed in ofloxacin-applied group. On the other hand, macrophages continued to express these cytokines in control group. From these results, the duration and amount of TGF-・1 expression is considered to affect the healing process. It is also considered that temporary increase of macrophages expressing TGF-・1 is playing an important role in the bone formation at the healing of periapical lesions.We also reported a case with halitosis associated with inappropriate endodontic treatment. We are now performing an experiment with clinical isolates from periapical periodontitis lesions, and the ability of coaggregation of the clinical isolates are being compared with the laboratory strains. We will reveal the host responses in periapical lesions. We did a work with probiotics, which changed the oral flora and improved the general health.

在根尖的病变，尤其是难治性的病变中，已知某些特定细菌会产生生物膜。他们也众所周知，可以使治疗更加困难。最近，据报道，梭菌核攻击与爆发有关（治疗后的急性期），并且经常讨论细菌的重要性。另一方面，宿主条件也被认为是爆发的重要因素。我们研究了TGF-1在根尖性病变发展中的作用。当将Loxacin蛋白施加到大鼠根尖细胞病变上时，巨噬细胞表现出TGF-・1增加并降低。在根尖区域观察到骨形成。在肺炎蛋白应用的组中观察到IL-1 ・和IL-1 ・・的表达降低。另一方面，巨噬细胞继续在对照组中表达这些细胞因子。从这些结果中，TGF-・1表达的持续时间和量被认为会影响愈合过程。还认为，表达TGF- 1的巨噬细胞的暂时增加在根尖性病变治愈时在骨形成中起着重要作用。我们还报道了一个病例，与牙内牙性治疗相关的胡尿病毒相关的病例。我们现在正在用临床周期性的临床范围和实验室的能力进行临床隔离的临床分离术，与临床隔离的实验相结合。宿主在根本病变中的反应。我们从事益生菌的工作，这改变了口腔菌群并改善了一般健康状况。

项目成果

歯周病原性細菌に対する免疫応答-マウス膿瘍モデルを用いた免疫組織学的検討-

对牙周病原菌的免疫反应 - 使用小鼠脓肿模型的免疫组织学研究 -

• 发表时间: 2008
• 作者: 吉兼 透;米田雅裕;他
• 通讯作者: 他

モチベーションに配慮しながら真性口臭症の治療を行った症例-口臭治療における歯科衛生士の役割

兼顾动机的真性口臭治疗案例——牙科保健员在口臭治疗中的作用

• 发表时间: 2008
• 期刊: 日本歯周病学会誌 50
• 作者: 鈴木奈央;米田雅裕;内藤徹;吉兼透;岩元知之;廣藤卓雄;峰真理子,米田雅裕,鈴木奈央,内藤徹,岡田一三,内田初美,吉兼透,島野裕一,岩元知之,廣藤卓雄
• 通讯作者: 峰真理子,米田雅裕,鈴木奈央,内藤徹,岡田一三,内田初美,吉兼透,島野裕一,岩元知之,廣藤卓雄

Case Presentation口臭の悩みを解消して患者さんに笑顔を-口臭の基礎知識とその治療における歯科衛生士の役割-

案例介绍缓解口臭问题，给患者微笑-口臭的基本知识以及牙科保健员在其治疗中的作用-

• 发表时间: 2008
• 期刊: 口臭DH style 2(24)
• 作者: 米田雅裕;峰真理子
• 通讯作者: 峰真理子

The relationship between alcohol consumption and oral malodor.

饮酒与口腔异味之间的关系。

• 发表时间: 2009
• 期刊: Int Dent J. 59(1)
• 作者: Nao Suzuki;Masahiro Yoneda;Toru Naito;Tomoyuki Iwamoto;Yosuke Masuo;Kazuhiko Yamada;Kazuhiro Hisama;Ichizo Okada;Takao Hirofuji
• 通讯作者: Takao Hirofuji

口臭治療のA to Z(3)真性口臭症の治療における歯科衛生士の役割

口臭治疗A to Z (3) 牙科保健员在真性口臭治疗中的作用

• 发表时间: 2008
• 期刊: デンタルハイジーン 28(9)
• 作者: 鈴木奈央;米田雅裕;内藤徹;吉兼透;岩元知之;廣藤卓雄;峰真理子,米田雅裕,鈴木奈央,内藤徹,岡田一三,内田初美,吉兼透,島野裕一,岩元知之,廣藤卓雄;米田雅裕,鈴木奈央,内藤徹,山田和彦,岡田一三,島野裕一,岩元知之,廣藤卓雄;米田雅裕,鈴木奈央,峰真理子,内藤徹,廣藤卓雄;鈴木奈央,米田雅裕,峰真理子,内藤徹,廣藤卓雄;峰真理子,米田雅裕,鈴木奈央,内藤徹,廣藤卓雄
• 通讯作者: 峰真理子,米田雅裕,鈴木奈央,内藤徹,廣藤卓雄